基于18S rRNA基因序列的我国马梨形虫分类学地位分析  

TAXONOMIC STATUS OF EQUINE PIROPLASMID ASSAYS BASED ON 18S RRNA GENE SEQUENCING IN CHINA

作　　者：罗金[1] 刘光远[1] 田占成[1] 谢俊仁[1] 张萍[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫原病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室农业部草食动物疫病重点开放实验室,甘肃兰州730046

出　　处：《动物分类学报》2011年第1期99-103,共5页Acta Zootaxonomica Sinica

基　　金：甘肃省科技重大专项计划(092NKDA031)

摘　　要：为从分子水平上阐明我国马梨形虫的种属分类特征。根据GenBank中登录的马梨形虫18SrRNA基因序列,在其高变区设计引物。PCR扩增获得大小分别为913bp、451bp的目的片段。将该片段克隆至pMD-18T载体后进行序列测定,并和GenBank上登录的其他地方株梨形虫18SrRNA基因序列进行同一性分析并构建系统发生树。分析显示,之前被称作马巴贝斯虫的虫种和泰勒虫虫种有着更密切的亲缘关系,在进化发育过程中处于同一种系,而与巴贝斯虫为明显的2个分支。我国驽巴贝斯虫肇源株与西班牙分离株(AY534883.1)仅有较小差异,其同源性高达91.8%。马泰勒虫肇源株与西班牙分离株(DQ287951.1,AY150064.2)同源性均高于91.4%。以上分析显示我国马梨形虫地方株和西班牙地方株亲缘关系最近,而与其他地方株差异相对较大。因此我国肇源株马梨形虫和西班牙地方株同属于Atbara8。There exsited a dispute on the taxonomic status of Theileria equi （T. equi）. To elucidate the taxonomic features of equine piroplasmid in China,We Designed primers in hypervariable region within 18S rRNA gene sequence of equine piroplasmid,and obtained a fragment with the length of 913 bp for T. equi and 451bp for Babesia caballi （B. caballi） by PCR,Subsequently the PCR products were ligated into pMD18-T vector and sequenced. The phylogenetic status of equine piroplasmid in China have been established with 18S rRNA gene sequences of others equine piroplasmid available in GenBank. The result of phylogenetic analysis indicated that T. equi clustered into the same branch with others theileria spp,which showed that T. equi should appropriately belong to Theileriidae. Furthermore,18S rRNA gene sequences of B. caballi are highly conserved across different geographical strains. The present study provided the molecular data for establishing the molecular diagnosis tool that used for investigating the epidemiology of equine piroplasmosis.

关 键 词：马梨形虫 马泰勒虫 驽巴贝斯虫 18S RRNA 系统发育 分类学 

分 类 号：Q959[生物学—动物学]