环氧合酶-2抑制剂对人舌鳞癌Tca8113/BLM细胞MDR1/P-gp表达的影响  

Effect of Cyclooxygenase-2 Inhibitor on Expression of MDR1 mRNA and P-glycoprotein in Human Tongue Cancer Drug-resistance Cell Line Tca8113/BLM

作　　者：李伟忠[1] 王晓燕[2] 霍秋菊[1] 

机构地区：[1]南方医科大学南方医院口腔科,广州510515 [2]南方医科大学南方医院病理科,广州510515

出　　处：《肿瘤防治研究》2011年第1期9-12,共4页Cancer Research on Prevention and Treatment

基　　金：广东省自然科学基金资助项目(06024396)

摘　　要：目的探讨环氧合酶-2抑制剂塞来昔布对人舌鳞癌耐药细胞系Tca8113/BLM细胞多药耐药基因和P-糖蛋白表达的影响。方法采用BLM(30μg/ml)反复24h暴露法处理人舌鳞癌细胞系Tca8113细胞,采用不同浓度的塞来昔布作用于Tca8113/BLM细胞,MTT法检测细胞增殖活性,流式细胞仪测定P-gp的表达水平,RT-PCR检测多药耐药基因mRNA的表达。结果塞来昔布显著抑制Tca8113/BLM细胞增殖、下调Tca8113/BLM细胞MDR1基因表达,其作用呈剂量依赖关系。结论塞来昔布以剂量依赖方式抑制人舌鳞癌耐药细胞系Tca8113/BLM细胞MD1l/P-gp表达,并抑制细胞增殖,这种作用可能与COX-2抑制剂增强抗癌药物对肿瘤细胞的杀伤作用有关。Objective To investigate the effect of celecoxib,a selective COX-2 inhibitor,on the expression of multidrug resistance gene（MDR1） and P-glycoprotein（P-gp） in human tongue cancer drug-resistance cell line Tca8113/BLM.Methods Human tongue squamous cell carcinoma cell line Tca8113 cells were repeatedly treated with BLM（30μg/ml） to establish drug-resistant cell line Tca8113/BLM.The inhibitory effect of celecoxib on Tca8113/BLM cell line was determined by MTT assay.The expression of P-gp and MRP were detected by flow cytometry and RT-PCR,respectively.Results The MTT results suggested that celecoxib inhibited the growth and proliferation of Tca8113/BLM cell lines in dose-dependent manner.Celecoxib treatment also resulted in significant down-regulated expression of MDR1 mRNA and P-gp.Conclusion Celecoxib shows a significant effect on inhibiting expression of MDR1 mRNA and P-gp in Tca8113/BLM cells,this is probably related to enhancing the lethal effects of anticancer agents against carcinoma cells.

关 键 词：环氧合酶-2抑制剂 鳞状细胞癌 P-糖蛋白 多药耐药基因 

分 类 号：R730.53[医药卫生—肿瘤] R739.86[医药卫生—临床医学]