人结肠癌裸鼠肝转移模型的建立  

Establishment and Comparison of Colon Cancer Liver Metastasis Models in Nude Mice

作　　者：袁岱岳[1] 郭忠英[2] 赵任[3] 黄宝玉[4] 梅广林[4] 朱建伟[4] 

机构地区：[1]南通大学第二附属医院普外科,江苏南通22600 [2]南通大学病理教研室 [3]上海瑞金医院普外科 [4]江苏省南通大学附属医院普外科微创组

出　　处：《肿瘤防治研究》2011年第1期28-30,共3页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金资助项目(30771126)

摘　　要：目的建立人结肠癌裸鼠肝转移模型,用于肿瘤转移防治的实验研究。方法 30只裸鼠随机分为两组,分别用肝脏注射法和脾脏注射法建立结肠癌肝转移模型,术后待裸鼠濒临死亡或自然死亡时,观察其生存天数,解剖裸鼠,观察腹腔内转移情况和肝脏成瘤的情况。结果两组裸鼠的肝脏成瘤率均为100%,生存期无明显差异。肝脏注射组裸鼠均于注射部位出现单一的巨大瘤结节,脾脏注射组裸鼠肝脏表面及切面见多发散在的转移瘤结节。两组的肝脏瘤组织细胞形态学相似,符合低分化腺癌的特征。结论经脾注射法建立裸鼠结肠癌肝转移模型比较符合结肠癌体内肝转移过程的规律,是一种简便有效的、重复性好的方法。Objective To develop a reliable colon cancer liver metastasis model in nude mice.Methods Thirty nude mice were divided into two groups randomly,which were the liver injection group and spleen injection group,respectively containing 15 nude mice.Colon cancer liver metastasis models were made by liver injection and spleen injection,respectively.The average survival time was recorded.The metastasis of nude mice was detected pathologically.Results Both of the incidence rates of liver metastasis in the two groups were 100%.Survival time of the two groups showed no statistics significance.Anatomical study showed that there appeared a single giant tumor nodule appeared at every injection site in the liver injection group while multiple metastasis appeared nodules in livers in the spleen injection group.The pathological results indicated that the tumor cells pattern of liver metastasis was similar in the two groups and was in accordance with poorly differentiated colon adenocarcinoma.Conclusion Intrasplenic injection of colon cancer cells,which closely mimic the natural metastatic process,is a reliable method for producing colon cancer liver metastasis in nude mice and should be proved valuable in experimental study.

关 键 词：结肠癌 肝转移 模型 裸鼠 

分 类 号：R735.35[医药卫生—肿瘤] R73-352[医药卫生—临床医学]