实时定量PCR检测成人ALL患者ZAP70基因的表达及意义  

Expression and Clinical Significance of ZAP70 in Adult Patients with Acute Lymphoblastic Leukemia Evaluated by Real-time Quantitative Polymerase Chain Reaction

作　　者：陈国枢[1] 徐兵[1] 宋小燕[1] 李洁[1] 郭绪涛[1] 周淑芸[1] 

机构地区：[1]南方医科大学南方医院血液科,广州510515

出　　处：《肿瘤防治研究》2011年第1期55-58,共4页Cancer Research on Prevention and Treatment

基　　金：广东省自然科学基金资助项目(5004737)

摘　　要：目的探讨成人急性淋巴细胞白血病(ALL)患者中Zeta链相关蛋白-70(ZAP70)基因的表达及其意义。方法构建实时荧光定量检测ZAP70基因表达的PCR技术,定量检测73例初治成人ALL患者ZAP70基因的表达水平。结果建立的实时定量PCR方法的标准曲线相关系数r>0.998。51例(69.8%)初治ALL患者可检出ZAP70基因表达。FAB各亚型中ZAP70基因表达水平差异无统计学意义(P>0.05),ZAP70基因表达水平的高低与初治ALL患者的免疫表型差异有统计学意义,T-ALL患者ZAP70基因表达水平显著高于B-ALL和T、B双表型ALL(P<0.01)。ZAP70基因表达水平的高低与ALL发病时外周血白细胞计数、血红蛋白浓度、血小板计数及骨髓白血病细胞比例无相关关系(P均>0.05)。初治ALL中ZAP70基因高低表达与患者化疗的完全缓解(CR)率差异无统计学意义,但在普通B-ALL组中,ZAP70高表达组的CR率(52.6%)显著低于ZAP70低表达组(85%)(P=0.041)。初治ALL中ZAP70基因高表达患者复发率明显高于低表达组(P=0.048)。结论 ZAP70基因高表达可能是ALL一个重要的预后不良因素。Objective To investigate the expressions and clinical significance of zeta-associated protein 70（ZAP70） gene in adult patients with acute lymphoblastic leukemia（ALL）.Methods Expressions of ZAP70 were detected by real-time fluorescent quantitative polymerase chain reaction（RQ-PCR） in 73 de novo adult ALL patients to analysis the relationship between its expressions and clinical effectiveness.Results The correlation co-efficiencies were over 0.998 for standard curves of RQ-PCR method.ZAP70 expressions were detected in 69.8% ALL patients.There were no statistical differences of ZAP70 gene expression levels in FAB subgroups.However,ZAP70 gene expression levels of T-ALL patients were significantly higher than that of B-ALL and biphenotypic ALL（P0.01） in immunophenotyping groups.The expressions level of ZAP70 gene were not correlated to peripheral white blood cell（WBC） counts,hemoglobin level,platelet counts and percentage of bone marrow blast cell at presentation.Statistical analyses showed that the difference of complete remission（CR） rates between high and low ZAP70 expressions in de novo ALL patients was not significant（P0.05）.In common B-ALL subgroup,the CR in the group with high expression level of ZAP70（52.6%）was significantly lower than that in the group with low expression level of ZAP70（85%）（P=0.041）.Patients with high expression levels of ZAP70（48.3% vs.21.7%,P=0.048）.ConclusionHigh expression of ZAP70 might be regarded as an important risk factor in ALL,which predicts a poor prognosis.

关 键 词：ZAP70 急性淋巴细胞白血病 PCR 预后 

分 类 号：R733.71[医药卫生—肿瘤]