大鼠舌癌癌变过程中琥珀酸脱氢酶的表达变化  

Changes in succinate dehydrogenase gene expression during 4-nitroquinoline 1-oxide induced rat tongue carcinogenesis.

作　　者：葛姝云[1] 娄佳宁[2] 周曾同[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院口腔黏膜科.口腔医学院.上海市口腔研究所.上海市口腔医学重点实验室,上海200011 [2]上海交通大学附属第一人民医院口腔科,上海200080

出　　处：《临床口腔医学杂志》2011年第1期20-22,共3页Journal of Clinical Stomatology

基　　金：国家自然科学基金青年项目(30700944);上海市重点学科建设项目基金资助(S30206)

摘　　要：目的:探讨大鼠口腔癌变过程中琥珀酸脱氢酶(Succinate dehydrogenase,SDH)的表达及其生物学意义。方法:0.002%4-硝基喹啉-1-氧化物(4NQO)饮水喂养Wistar大鼠9-32周建立大鼠舌癌变模型,用实时荧光定量PCR法检测30例大鼠舌癌变过程不同病理阶段组织中SDH的表达水平。结果:轻度异常增生、中度异常增生、重度异常增生。鳞癌组织病理分级中,中度异常增生、重度异常增生、鳞癌组织中SDH表达显著低于正常黏膜(P<0.05或P<0.01)。SDH表达与组织的病理学分级相关(P<0.01)。结论:SDH基因表达与口腔癌发生发展相关,三羧酸循环在口腔黏膜癌变过程中可能起到重要作用。Objective：To observe the different expression of Succinate dehydrogenase（SDH） during oral carcinogenesis,analyze the relationship between the abnormal expression of SDH,and the occurrence and development of oral carcinogenesis.Method：Real-time quantitative PCR was employed to detect the gene expression of SDH in 38 rat tongue carcinogenesis specimens induced by 4-nitroquinoline 1-oxide.Result：with the progress of tongue carcinogenesis,the expression of SDH was down-regulated.in normal,Mild epithelial dysplasia、Moderate epithelial dysplasia、severe epithelial dysplasia and squamous cell carcinoma（SCC）.There was significant differences between normal and Moderate epithelial dysplasia、severe epithelial dysplasia and SCC,P 0.01 or P 0.05）.There was a significant correlation between the decreased expression levels of SDH gene and histopathological grade（P 0.01）.Conclusion：The down expression of SDH gene may be correlated to the occurrence and the development of oral carcinoma,suppressed Tricarboxylic acid cycle might in the development or progression of oral cancer.

关 键 词：口腔癌变 琥珀酸脱氢酶 实时荧光定量PCR 4-硝基喹啉-1-氧化物 

分 类 号：R781.5[医药卫生—口腔医学] Q786[医药卫生—临床医学]