VEGF对兔骨髓细胞分化形成破骨细胞的诱导作用  

Effect of VEGF on osteoclasts formation from bone marrow cells in rabbit

作　　者：孙华 宋开茂[2] 王敏 王小琼[3] 

机构地区：[1]解放军兰州军区疗养院第二疗养区口腔科,陕西西安710600 [2]解放军273医院口腔科,新疆库尔勒841000 [3]西安建筑科技大学医院口腔科,陕西西安710055

出　　处：《牙体牙髓牙周病学杂志》2011年第1期11-13,共3页Chinese Journal of Conservative Dentistry

摘　　要：目的:观察血管内皮生长因子(VEGF)对兔骨髓细胞分化为破骨细胞的诱导作用。方法:应用终未浓度为5 ng/mL的人重组rhVEGF体外诱导培养兔骨髓细胞,并与骨片共同培养,分别于诱导培养后3、6、9 d用抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphatase,TRAP)染色进行破骨细胞鉴定计数和骨片吸收陷窝数目的统计。结果:诱导培养3 d,实验组与对照组的破骨细胞数和骨吸收陷窝数无显著性差异(P>0.05),而随着诱导培养时间延长,实验组两者的数量明显增加,在诱导培养6 d和9 d时,均明显高于对照组,差异有统计学意义(P<0.05)。结论:VEGF具有体外诱导骨髓细胞形成破骨细胞的作用。AIM：To observe the inducing effect of recombinant human vascular endothelial growth factor（rhVEGF） on osteoclasts differentiation from rabbit bone marrow cells.METHODS：Rabbit bone marrow cells were harvested and cultured in the presence or absence of 5ng/mL rhVEGF.On day 3,6 and 9,the cells were stained with tartrate-resistant acid phosphatase（TRAP） and bone absorption lacunas were counted.RESULTS：On day 3 after rhVEGF induction,no difference was found in the number of TRAP-positive cells and absorption lacunas（P0.05）.However,the number of TRAP-positive cells and absorption lacuna were significantly increased on day 6 and day 9 post-rhVEGF induction.CONCLUSION：VEGF can induce osteoclasts differentiation in vitro.

关 键 词：人重组血管内皮生长因子 兔 破骨细胞 

分 类 号：R780.2[医药卫生—口腔医学]