ABCB1、ABCG2与食管癌患者多药耐药的关系  

Research of esophageal cancer multi-drug resistance

作　　者：刘亮[1] 左静[2] 赵丽[1] 左连富[1] 郭建文[1] 王静[1] 刘江惠[1] 

机构地区：[1]河北医科大学第四医院肿瘤研究所流式细胞室,河北石家庄050011 [2]河北医科大学第四医院肿瘤内科,河北石家庄050011

出　　处：《中国老年学杂志》2011年第1期15-17,共3页Chinese Journal of Gerontology

基　　金：河北省自然科学基金资助(No.C2007001060);河北省普通高等学校强势特色学科肿瘤学建设经费资助(冀教高〔2005〕52号)

摘　　要：目的探讨多药耐药蛋白三磷酸腺苷结合转运蛋白G2(ABCG2)蛋白和三磷酸腺苷结合转运蛋白B1(ABCB1)在食管癌组织中的表达及其意义。方法选用80例食管癌患者手术切除标本,采用流式细胞术(FCM)方法检测80例原发性食管癌患者的癌组织、癌旁组织(距离癌2 cm)及食管切缘正常黏膜(距离癌5 cm以上)ABCG2及ABCB1蛋白的表达量。结果 ABCG2及ABCB1蛋白在食管癌组织中有较高程度的表达,表达量分别为(467.30±37.85)、(444.79±22.83),而正常食管黏膜中表达量分别为(418.41±21.70)、(401.35±16.09);其在食管癌中的表达显著高于正常黏膜(P<0.01)。ABCG2蛋白及ABCB1蛋白在低分化鳞癌中的表达量显著高于中高分化鳞癌(P<0.05),在侵及纤维膜的癌组织中表达显著高于未侵及纤维膜的癌组织(P<0.05),在有淋巴结转移的癌组织中表达显著高于无淋巴结转移癌组织(P<0.01),在年龄和性别间表达无显著性差异(P>0.05)。结论 ABCG2及ABCB1参与食管癌多药耐药的形成,对于临床上食管癌的化疗具有一定的指导意义。Objective To explore the mechanism of esophageal cancer multi-drug resistance and detect the relationships between the expression of ABCB1,ABCG2 in esophageal cancer and esophageal cancer multi-drug resistance.Methods 80 cases esophageal cancer tissues were taken,including cancer,atypical hyperplasia and normal tissues.Expressions of ABCB1 and ABCG2 protein were examined by flow cytometry.Results The expressions of ABCG2,ABCB1 protein in esophageal cancer was higher than those of normal esophageal tissues significantly（P0.01）. The expressions of ABCG2,ABCB1 protein in poorly cell differentiation,fibrous membrane invasion,lymph node metastasis positive esophageal cancer was higher than those of well/ moderately cell differentiation,fibrous membrane untouched,lymph node metastasis negative tissues significantly（P0.05）.The expressions of ABCG2,ABCB1 protein was no significant difference in gender and age（P0.05）.Conclusions The high expression of ABCG2,ABCB1 protein in esophageal cancer could participate in the development of esophageal cancer and multi-drug resistance.

关 键 词：食管癌 三磷酸腺苷结合转运蛋白G2 三磷酸腺苷结合转运蛋白B1 多药耐药 流式细胞术 

分 类 号：R735.1[医药卫生—肿瘤]