叉头样转录因子3及转化生长因子β在小儿肾母细胞瘤中的表达和意义  

Expressions and clinical significance of FOXP3 mRNA and TGF-β mRNA in nephroblastoma

作　　者：刘娣[1] 许晓群[1] 陈雪梅[2] 张建华[1] 陈志鸿[3] 于启海[3] 王郡甫[1] 

机构地区：[1]山东省医学科学院基础医学研究所山东省现代医用药物与技术重点实验室,济南250062 [2]山东大学第二医院 [3]山东省立医院

出　　处：《中华小儿外科杂志》2011年第1期40-43,共4页Chinese Journal of Pediatric Surgery

基　　金：山东省自然科学基金（编号：2R2009CM036）;山东省中青年科学家奖励基金（编号：BS2009SW007）

摘　　要：目的 探讨叉头样转录因子3（FOXP3）及转化生长因子β（TGF-β）mRNA在小儿肾母细胞瘤中的表达和临床意义.方法 采用RT-PCR方法检测30例肾母细胞瘤组织、30例瘤旁组织和3例正常肾组织中FOXP3及TGF-βmRNA的表达情况,观察两者表达与临床分期的关系.结果 与瘤旁组织和正常组织比较,FOXP3及TGF-βmRNA在肿瘤组织中表达均上升,为0.706±0.107、0.667±0.121,差异具有统计学意义（P＜0.001）;二者mRNA在Ⅰ期肿瘤中的表达强度为0.535±0.086、0.564±0.050均低于Ⅱ期的0.741±0.074、0.704±0.067和Ⅲ期的0.843±0.053、0.734±0.085,差异具有统计学意义（P＜0.001）;FOXP3和TGF-βmRNA表达强度呈明显正相关（r=0.749,P＜0.001）.结论 小儿肾母细胞瘤组织中,FOXP3及TGF-βmRNA表达增高,可能与肾母细胞瘤的发展和转移有关.Objective To investigate the expression levels and the clinical significance of FOXP3and TGF-β in nephroblastoma. Methods The expression levels of FOXP3 and TGF-β were evaluated using RT-RCR in 30 nephroblastoma,30 adjacent tissues and 3 normal tissues. Results The expression levels of FOXP3 and TGF-β in nephroblastoma tissues were higher than adjacent and normal tissues（P＜0. 001）. Compared to the stage Ⅱ and Ⅲ the difference of the expression levels of FOXP3and TGF-β in stage Ⅰ were significant in nephroblastoma tissues（P＜0. 001）. There was a positive correlation between FOXP3 and TGF-β. Conclusions The expression levels of FOXP3 and TGF-β were higher in tumor tisssues, suggesting that it may be related to metastasis and progression of the nephroblastoma.

关 键 词：肾母细胞瘤 转化生长因子Β 逆转录聚合酶链反应 

分 类 号：R737[医药卫生—肿瘤]