猪细小病毒感染PK-15细胞抗病毒相关因子转录变化的分析  

The Analysis of Antiviral Cytokines Transcriptional Profiles of PK-15 Cell Cultures Following Infection with Porcine Parvovirus

作　　者：李厚伟[1] 魏战勇[2] 尹海燕[3] 陈红英[2] 李金磊[1] 韩志涛[1] 崔保安[1] 

机构地区：[1]河南省动物性食品安全重点实验室,郑州450002 [2]河南农业大学牧医工程学院,郑州450002 [3]河南省农业科学院农业质量标准与检测技术研究中心,郑州450002

出　　处：《畜牧兽医学报》2011年第1期48-55,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：河南省杰出人才创新基金项目(0621002100)

摘　　要：为了解猪细小病毒(PPV)感染后引起干扰素及其相关细胞因子的反应,探讨宿主—病毒之间的作用关系,作者运用荧光定量PCR技术,测定和分析PPV感染PK-15细胞引起的病毒DNA量的变化和细胞因子IFN-β、IFN-γ、IFNAR-1、IFNAR-2、MHC-Ⅰ、MHC-Ⅱ、MX1、iNOS、2-5AS、RNase L和IRF-3的转录水平。结果显示,PPV感染PK-15细胞12h病毒开始大量迅速增殖,48h达到最高峰;PPV感染后可引起PK-15细胞中IFN-β、IFN-γ、IFNAR-1、IFNAR-2、MHC-Ⅰ、MHC-Ⅱ、Mx1、iNOS、2-5AS、RNase L和IRF-3的转录量显著增加,其中Mx1基因在24h转录量达到8 423倍。猪细小病毒感染可引起PK-15细胞抗病毒相关因子转录增加。In order to survey the host inflammatory responses,particularly the antiviral cytokines responses of Porcine Parvovirus（PPV） infection,and to investigate the host-PPV interaction,Porcine Kidney-15 cells（PK-15） were infected by PPV.Then the viral DNA was measured and analyzed using real-time PCR.The transcript level of cytokines（IFN-β,IFN-γ,IFNAR-1,IFNAR-2,MHC-Ⅰ,MHC-Ⅱ,MX1,NOS,2-5AS,RNase L and IRF-3） were detected by real-time PCR too.The results showed that PPV proliferated rapidly in PK-15 cell 12 hours after infection,and reached peak 48 hours after infection;the transcription levels of IFN-β,IFN-γ,IFNAR-1,IFNAR-2,MHC-Ⅰ,MHC-Ⅱ,MX1,iNOS,2-5AS,RNase L and IRF-3 were increased obviously,and the transcription level of Mx1 gene was increased 8 423 times at 24 hours after infection.The results revealed that the level of antiviral cytokines increased in PPV infected PK-15 cell.

关 键 词：猪细小病毒 细胞因子 PK-15细胞 转录时相 

分 类 号：S852.659.2[农业科学—基础兽医学]