B淋巴增殖性疾病的SOX11、cyclin D1、cyclin D2和cyclin D3的表达研究  

The expression of SOX11, cyclin D1, cyclin D2 and cyclin D3 in B-cell lymphocytic proliferative diseases

作　　者：曹鑫[1] 范磊[1] 方成[1] 朱丹霞[1] 王冬梅[1] 董华洁[1] 刘玲[1] 王银花[1] 徐卫[1] 李建勇[1] 

机构地区：[1]南京医科大学第一附属医院、江苏省人民医院血液科,南京210029

出　　处：《国际输血及血液学杂志》2011年第1期4-9,共6页International Journal of Blood Transfusion and Hematology

基　　金：国家自然科学基金资助项目（30871104,30971296,30971295）;江苏省自然科学基金资助项目（BK2010584）;江苏省医学领军人才基金资助项目（LJ200623）;江苏省医学重点人才基金资助项目（RC2007042）;江苏省“青蓝工程’’中青年学术带头人培养对象项目;江苏省“六大人才高峰”资助项目

摘　　要：目的 探讨各B淋巴增殖性疾病（B-LPD）中SOX11、cyclin D1、cyclin D2和cyclin D3表达的差异和相关性,以及与慢性淋巴细胞白血病（CLL）患者临床特征的关系.方法 采用实时定量逆转录PCR（qRT-PCR）技术检测154例B-LPD患者与12例健康对照SOX11、cyclin D1、cyclin D2和cyclin D3基因的表达水平;应用免疫组织化学技术对15例B-LPD患者组织标本进行SOX11蛋白核表达水平的检测.结果 套细胞淋巴瘤（MCL）患者SOX11表达水平较高,与CLL、弥漫大B细胞淋巴瘤（DLBCL）、毛细胞白血病（HCL）、脾边缘区淋巴瘤（SMZL）和健康对照的差异存在统计学意义（P1=0.002、P2=0.017、P3=0.015、P4=0.008、P5=0.008）;MCL患者cyclin D1的表达明显高于CLL、DLBCL、HCL、SMZL和健康对照（P1＜0.001、P2=0.008、P3=0.020、P4＜0.001、P5=0.005）;DLBCL患者的cyclin D1和cyclin D2表达高于SMZL患者（P1=0.049,P2=0.005）;SOX11的表达与cyclin D1、cyclin D2和cyclin D3呈显著正相关（r1=0.444,P1＜0.001;r2=0.279,P2＜0.001;r3=0.425,P3＜0.001）;15例患者标本SOX11核蛋白表达的检测结果显示,4例MCL中有3例为阳性,1例阴性;其余12例均为阴性,包括7例DLBCL、2例粘膜相关组织（MALT）淋巴瘤、2例SMZL.CLL患者中,ZAP70阳性患者SOX11的表达水平明显高于ZAP70阴性的患者（P=0.021）.结论 MCL患者SOX11和cyc1in D1 mRNA的表达水平较高,并特异性的表达SOX11核蛋白.Objective To investigate the expression levels of the SOX11 and cyclin D isoforms in Bcell lymphocytic proliferative disease （B-LPD）. Methods We assessed the expressions of SOX11, cyclins D1, cyclin D2, cyclin D3 mRNA using real-time quantitative reverse transcriptase-polymerase chain reaction （qRT-PCR） and SOX11 protein using immunohistochemistry. Results Data showed the transcriptional level of SOX11 was higher for the mantle cell lymphoma （MCL） samples compared with the groups of chronic lymphocytic leukemia （CLL）, diffuse large B cell lymphoma （DLBCL）, hairy cell leukemia （HCL）, splenic marginal zone lymphoma （SMZL） and healthy collators （P1 =0. 002, P2 =0. 017, P3 =0. 015, P4 =0. 008,P5 = 0. 008, respectively）. Also MCL showed higher transcript level of cyclin D1 than CLL, DLBCL,HCL, SMZL and healthy collator group （P1 〈 0. 001, P2 = 0. 008, P3 = 0. 020, P4 〈 0. 001, P5 = 0. 005,respectively）. The cyclin D1 and cyclin D2 expression levels were both higher in DLBCL group than in SMZL group （P1 =0. 049, P2 =0. 005, respectively）. The SOX11 expression level was highly related with the expression levels of cyclin D1, cyclin D2 and cyclin D3 （r1 = 0. 444, P1 〈0. 001 ; r2 = 0. 279, P2 〈0. 001; r3 =0. 425, P3〈0. 001, respectively）. Moreover, 3 out of 4 MCL patients showed nuclear staining forSOX11, while other 11 B-LPD examples were negative, including 7 cases of DLBCL, 2 cases of mucosaassociated lymphoid tissue （MALT） lymphoma, 2 cases of SMZL. Futhermore, we also found the ZAP70-positive CLL patients had higher SOX11 expression levels than those ZAP70-negative CLL patients （P1 =0. 021）. Conclusion It was revealed that MCL patients had a higher expression levels of SOX11 and cyclin D1 mRNA, and specially expressed nuclear SOX11 protein.

关 键 词：B淋巴增殖性疾病 MRNA SOX11 CYCLIN D1 CYCLIN D2 CYCLIN D3 

分 类 号：R551.2[医药卫生—血液循环系统疾病]