2型糖尿病脑梗死患者血清MMP-2、VEGF与糖代谢异常的相关性  

The relationship between serum MMP-2,VEGF and abnormal glyco-metabolism in cerebral infarction patients with type 2 diabetes mellitus

作　　者：陈欢[1] 朱梅佳[2] 胥文娟[1] 马高亭[1] 赵张宁[2] 

机构地区：[1]山东大学医学院,济南250012 [2]山东大学附属千佛山医院神经内科,济南250014

出　　处：《山东大学学报（医学版）》2011年第1期23-27,共5页Journal of Shandong University:Health Sciences

基　　金：山东省科技攻关计划资助项目(2007GGWZ02056)

摘　　要：目的探讨血清基质金属蛋白酶-2（MM2-2）、血管内皮生长因子（VEOr；）在2型糖尿病脑梗死患者脑组织损伤及修复过程中的作用。方法选取30名2型糖尿病脑梗死患者（DMCI组）、30名单纯脑梗死患者（CI组）、20名健康体检者作为对照组（C组）。其中DMCI组：男11名，女19名，60—80岁，平均71．2岁；CI组：男12名，女18名，60～80岁，平均70．3岁；c组：男9名，女11名，60—80岁，平均69．0岁。采用酶联免疫吸附法检测DM—CI组、CI组发病1、7d及对照组体检时血清中MMP-2、VEGF的含量。结果DMCI组发病1、7d血清MMP一2水平均高于CI组和c组（P〈0．05）；发病1d血清VEGF水平与cI组比较差异无统计学意义（P〉0．05），但显著高于c组（P〈0．01）；发病7d血清VEGF水平显著低于cI组（P〈0．01），但显著高于C组（P〈0．01）。DMCI组、cI组发病7dMMP一2、VEGF水平均显著高于发病1d（P〈0．05）。DMCI组血清MMP-2水平与FBG、HbAlc含量呈显著正相关（r=0．829，P=0．000；r=0．855，P=0．000）；血清VEGF水平与FBG、HbAlc含量呈显著负相关（r=-0．501，P=0．005；r=-0．528，P=0．003）；血清MMP-2水平与VEGF水平呈显著负相关（r=-0．586，P=0．001）。结论2型糖尿病脑梗死患者不同时间段血清MMP-2、VEGF水平与糖代谢异常有显著相关性，MMP-2、VEGF参与糖尿病脑梗死的病理生理过程。Objective To study the role of matrix metalloproteinase-2 （MMP-2） and vascular endothelial growth factor （VEGF） in the injury and repair of brain tissue in cerebral infarction patients with type 2 diabetes mellitus. Methods 60 patients were enrolled in this study, including 30 cerebral infarction patients with type 2 diabetes mellitus （DMCI group： 11 men, 19 women; mean age 71.2 years old, from 60 to 80 yrs）, and 30 simple cerebral infarction patients （CI group： 12 men, 18 women; mean age 70.3 years old, from 60 to 80 years）. 20 healthy subjects were recruited as a control （ C group ： 9 men, 11 women ; mean age 69.0 years old, from 60 to 80 yrs）. Enzyme-linked immunosorbent assay （ELISA） was used to measure the levels of serum MMP-2 and VEGF in the DMCI group and the CI group on the first and 7th days after onset. The same protocol was applied in the control group. Results On the first day and 7th days after the onset of infarction, the level of serum MMP-2 was significantly higher in the DMCI group compared with the CI and control group （P 〈 0.05）. The DMCI group showed the same serum VEGF level as the CI group （ P 〉 0.05）on the first day after the onset, which was considerably higher than the C group （P 〈0.01 ）. On the seventh day, the level of serum VEGF was significantly lower in the DMCI group than the CI group （P 〈0.01 ）, but still higher than C group （P 〈0.01 ）. The levels of serum MMP-2 and VEGF on the 7th days were significantly higher than those on the first day in both the DMCI group and the CI group （ P 〈 0.05 ）. In the DMCI group, the level of serum MMP-2 was positively correlated with fasting blood sugar （ FBS ） and HbAlc （ r = 0. 829, P = 0. 000 ; r = 0. 855, P = 0. 000 ）, however, VEGF was negatively correlated with both （r= -0. 501, P=0. 005; r= -0. 528, P =0.003）. Also, the level of serum MMP-2 was negatively correlated with, VEGF （ r = - 0. 586, P = 0.001 ）. Conclusion There are significant correlations among the

关 键 词：脑梗死 糖尿病 2型 MMP-2 VEGF 

分 类 号：R743.32[医药卫生—神经病学与精神病学]