围植入期暴露二硫化碳对小鼠子宫组织白血病抑制因子表达的影响  

Effect of carbon disulfide exposure during peri-implantation on expression of leukaemia inhibitory factor in the uterus of mice

作　　者：吴艳玲[1] 胡程霞[1] 张炳珍[1] 孙淑爱[1] 王志萍[1] 

机构地区：[1]山东大学公共卫生学院流行病学与卫生统计学研究所,济南250012

出　　处：《山东大学学报（医学版）》2011年第1期124-128,共5页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助项目(30671745;81072275);山东省自然科学基金资助项目(Y2006C39)

摘　　要：目的观察二硫化碳(CS2)对孕鼠子宫组织白血病抑制因子(LIF)表达水平的影响,探讨CS2致胚胎毒性的作用机制。方法健康昆明种孕鼠随机分为D3(孕第3天)、D4、D5、D6染毒组和对照组,每组又根据各自的染毒时点分别设相应的观察终点(记为D4、D5、D6、D7、D9)。染毒组腹腔注射631.4mg/kg剂量的CS21次,对照组给予橄榄油。在相应观察终点结束实验,观察CS2对孕鼠和胚胎植入的影响,并应用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)和蛋白免疫印迹法(Western blotting)测定子宫组织中LIF表达水平。结果胚胎围植入期不同时点暴露CS2均可导致孕鼠D9胚胎植入数目减少,D3、D4、D5、D6染毒组胚胎植入数目分别比对照组减少了81.1%、92.7%、91.5%和66.9%,差异具有统计学意义(P<0.05);围植入期不同时点暴露CS2可导致孕鼠子宫组织中LIF在各染毒组相应的第一个观察终点低表达,分别较对照组降低了43.8%、54.3%、38.7%和22.6%,差异具有统计学意义(P<0.01);胚胎植入数目与子宫组织LIF表达水平呈正相关。结论胚胎围植入期暴露CS2可导致孕鼠子宫组织中LIF表达水平降低,这可能是CS2致胚胎毒性的分子机制之一。Objective To observe the effect of carbon disulfide（CS2） on expression of leukaemia inhibitory factor（LIF） in the uterus of pregnant mice and to explore the mechanism of embryo toxicity induced by CS2.Methods At the phase of embryonic peri-implantation which was subdivided into D3（the third day in gestation）,D4,D5 and D6 phases,mice were intraperitoneally exposed to CS2（in a dosage of 631.4 mg/kg,and a volume of 0.1 mL/10 g body weight） once,mice were intraperitoneally exposed to olive oil as the controls.All indicators were obtained at the corresponding end points located（denoted as D4,D5,D6,D7 and D9） in accordance with the exposure time.Effects of CS2 on pregnant mice and the number of implanted embryos were observed,and expression of LIF in the uterus was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis（SDS-PAGE） and Western blot.Results ① The number of implanted embryos significantly decreased after exposure at each phase during peri-implantation compared with the controls（decreasing 81.1%,92.7%,91.5% and 66.9%,respectively,all P 〈0.05）.② Expression of LIF in the uterus obviously declined at the first endpoint in accordance with their exposure time（P 〈0.01）,and compared with the controls,the reduced rates were 43.8%,54.3%,38.7% and 22.6%,respectively.③ The number of embryos was positively related to the level of LIF expressed in the uterus of mice.Conclusion Exposure to CS2 during peri-implantation disturbs expression of LIF in the uterus of pregnant mice,which might be one of the important mechanisms contributing to embryo toxicity induced by CS2.

关 键 词：二硫化碳 胚胎毒性 胚胎植入 白血病抑制因子 

分 类 号：R113[医药卫生—公共卫生与预防医学]