A new tumor necrosis factor（TNF）-α regulator,lipopolysaccharides-induced TNF-α factor,is associated with obesity and insulin resistance  

作　　者：JI Zhen-zhong DAI Zhe XU Yan-cheng 

机构地区：[1]Department of Endocrinology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China

出　　处：《Chinese Medical Journal》2011年第2期177-182,共6页中华医学杂志（英文版）

基　　金：This work was supported by a grant from the National Natural Science Foundation of China （No. 30871188）.

摘　　要：Background Tumor necrosis factor （TNF）-α plays an important role in mediating inflammatory state in obesity and related disorders.Lipopolysaccharides （LPS）-induced TNF-α factor （LITAF） is recently verified as a regulator of TNF-α and other inflammatory cytokines,and maybe act as a transcriptional factor.The aim of this study was to confirm the association between LITAF and obesity and insulin resistance.Methods Forty-seven subjects with a wide range of body mass index （BMI） were included.Subjects were divided intothree groups according to the criteria of normal weight,overweight and obese.Anthropometrics and metabolic profile were tested for all the subjects.Peripheral monocytes were isolated and purified.LITAF transcription was detected by real time PCR,and the protein expression in whole cell and nucleus extracts was detected by Western blotting analysis;transcriptional activity of LITAF was detected by ELISA like assay using a probe containing the DNA binding sequence of LITAF.Plasma TNF-α and interleukin （IL）-6 concentrations were determined with ELISA kit.Results The LITAF mRNA and protein expression in whole cell were higher in overweight （P 〈0.05） and obese group （P 〈0.05） compared with that in normal weight group.The LITAF protein expression in the nucleus and transcriptional activity could not be detected.LITAF protein expression was positively correlated with BMI （r=0.541,P 〈0.001）,waist circumference （r=0.391,P=0.007）,the homeostasis model assessment for insulin resistance （r=0.372,P=0.011） and fasting insulin levels （r=0.359,P=0.013）.As a regulator of inflammatory cytokines,LITAF protein expression was positively correlated with plasma TNF-α （r=0.621,P=0.002） and IL-6 （r=0.407,P=0.039） concentration.Multiple variant regression analysis indicated that BMI （P=0.002） and waist circumference （P=0.017） were independent predictors of LITAF protein expression.Conclusions LITAF is associated with obesity and insulin resistance,as well as iBackground Tumor necrosis factor （TNF）-α plays an important role in mediating inflammatory state in obesity and related disorders.Lipopolysaccharides （LPS）-induced TNF-α factor （LITAF） is recently verified as a regulator of TNF-α and other inflammatory cytokines,and maybe act as a transcriptional factor.The aim of this study was to confirm the association between LITAF and obesity and insulin resistance.Methods Forty-seven subjects with a wide range of body mass index （BMI） were included.Subjects were divided intothree groups according to the criteria of normal weight,overweight and obese.Anthropometrics and metabolic profile were tested for all the subjects.Peripheral monocytes were isolated and purified.LITAF transcription was detected by real time PCR,and the protein expression in whole cell and nucleus extracts was detected by Western blotting analysis;transcriptional activity of LITAF was detected by ELISA like assay using a probe containing the DNA binding sequence of LITAF.Plasma TNF-α and interleukin （IL）-6 concentrations were determined with ELISA kit.Results The LITAF mRNA and protein expression in whole cell were higher in overweight （P 〈0.05） and obese group （P 〈0.05） compared with that in normal weight group.The LITAF protein expression in the nucleus and transcriptional activity could not be detected.LITAF protein expression was positively correlated with BMI （r=0.541,P 〈0.001）,waist circumference （r=0.391,P=0.007）,the homeostasis model assessment for insulin resistance （r=0.372,P=0.011） and fasting insulin levels （r=0.359,P=0.013）.As a regulator of inflammatory cytokines,LITAF protein expression was positively correlated with plasma TNF-α （r=0.621,P=0.002） and IL-6 （r=0.407,P=0.039） concentration.Multiple variant regression analysis indicated that BMI （P=0.002） and waist circumference （P=0.017） were independent predictors of LITAF protein expression.Conclusions LITAF is associated with obesity and insulin resistance,as well as i

关 键 词：OBESITY insulin resistance lipopolysaccharides-induced TNF-α factor TNF-Α 

分 类 号：Q78[生物学—分子生物学] Q578