Hsa-miR-133a对人乳腺癌细胞侵袭和迁移的影响  

Effect of Hsa-miR-133a on breast cancer cell invasion and migration potential

作　　者：王超群[1] 吴正升[1] 张瑰红[1] 项茹[1] 朱涛[2] 徐晓春[1,3] 吴强[1] 

机构地区：[1]安徽医科大学病理学教研室/第二附属医院病理科,合肥230032 [2]中国科学技术大学生命科学院,合肥230027 [3]美国UTMD Andeson Cancer Center

出　　处：《临床与实验病理学杂志》2011年第1期15-18,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金项目(30873047);安徽高校省级自然科学研究重点项目(KJ2009A162);安徽医科大学第二附属医院引进人才基金(2009/3)

摘　　要：目的探讨Hsa-miR-133a(miR-133a)对人乳腺癌细胞侵袭、迁移和增殖的影响,并初步分析miR-133a影响乳腺癌细胞侵袭及迁移的可能机制。方法用脂质体介导的转染方法将miR-133a阻遏物(miR-133a inhibitors)转染人乳腺癌细胞株MCF-7,以inhibitor negative control(inhibitor NC)作为阴性对照。通过MTS试剂盒和Transwell侵袭实验检测细胞增殖能力和侵袭力;采用Transwell迁移实验及划痕试验检测细胞迁移能力;再利用生物信息学方法预测miR-133a的靶基因,并对其靶基因进行基因功能分析。结果 (1)miR-133a inhibitors组与inhibitor NC组间细胞增殖活性差异无显著性(P>0.05);(2)划痕后miR-133a inhibitors组细胞迁移能力比inhibitor NC组明显增强(P<0.05);(3)Transwell侵袭及迁移实验显示转染miR-133a inhibitors后,MCF-7细胞的侵袭及迁移能力均明显增强(P<0.01,P<0.05);④生物信息学方法预测miR-133a的靶基因中,部分发挥了促进细胞侵袭、迁移的生物学功能。结论 (1)MiR-133a对人乳腺癌细胞的侵袭及迁移能力可能存在负性调控作用,可能成为乳腺癌治疗的潜在候选靶点;(2)MiR-133a可能通过多种靶基因发挥其对肿瘤的调控作用。Purpose To investigate the effect of miR-133a on breast cancer cell invasion,migration and proliferating potential,and to explore its possible targets and mechanism.Methods Human breast cancer MCF-7 cells were transfected with Hsa-miR-133a inhibitors by Lipofectamine to reduce the activity of miR-133a.MCF-7 cells transfected with inhibitor negative control（inhibitor NC） were cultured as negative control.Cell proliferation and invasion potential were evaluated by MTS kit and transwell invasion assay.Cell migrating ability of MCF-7 was detected by transwell migration assay and wound-healing assay.Then the possible target genes of miR-133a were forecasted by bioinformatics tools,and the function of these genes was analyzed.Results Cells transfected with miR-133a inhibitors revealed no significant change in growth compared to the inhibitor NC transfectants（P0.05）.Remarkable enhancement of cell migration was observed in miR-133a inhibitors transfectant after the scratch（P0.05）.Transfection of miR-133a inhibitors into MCF-7 cells led to a significant increase in cell invasion and migration detected by transwell invasion and migration assay（P0.01 and P0.05）.The possible target genes of miR-133a were forecasted by bioinformatics tools,and part of the target genes played a biological function of promoting cell invasion,migration.Conclusions MiR-133a may negatively regulate the invasion and migration potential of breast cancer cells and may be a potential candidate target for breast cancer treatment.MiR-133a may play a function of regulation of tumor development through some target genes.

关 键 词：乳腺肿瘤 MIRNA miR-133a TRANSWELL 侵袭 迁移 

分 类 号：R737.9[医药卫生—肿瘤] R394.2[医药卫生—临床医学]