自体预变性腓总神经调节大鼠受损视神经的细胞凋亡和增殖及其基因表达  

Predegenerated common peroneal nerve regulates cells apoptosis/proliferation and their gene expressions in injured rat optic nerve

作　　者：袁建明[1] 蔺海燕[1] 刘芳[1] 郭金萍[1] 张玲珍[2] 许家军[1] 

机构地区：[1]第二军医大学解剖学教研室,上海200433 [2]上海长征医院实验诊断科,上海200003

出　　处：《解剖学报》2011年第1期1-6,共6页Acta Anatomica Sinica

基　　金：国家重点基础研究发展计划资助项目(2011CB707502);国家自然科学基金资助项目(30271392)

摘　　要：目的研究预变性自体腓总神经对受损大鼠视神经细胞凋亡和增殖的作用。方法 115只成年雄性大鼠分为正常组、损伤组、移植组,分别用HE染色、流式细胞仪、免疫印迹、基因芯片、荧光定量PCR检测视神经细胞数量、增殖和凋亡;增殖性细胞核抗原(PCNA)表达;增殖、凋亡相关基因表达谱的变化。结果损伤组视神经细胞较正常组增加,移植组较损伤组增加。损伤组与正常组相比,凋亡细胞明显增加,增殖细胞明显减少;移植组与损伤组相比,凋亡细胞减少,增殖细胞增加。与正常组相比,损伤组PCNA表达下调,移植组较损伤组明显上调。损伤组与正常组相比,差异表达的细胞凋亡和增殖相关基因37条;移植组与损伤组相比,差异表达的相应基因3条。结论视神经损伤致远侧段细胞凋亡,增殖减弱,移植自体预变性腓总神经能减轻细胞凋亡、促进细胞增殖,并伴相应的基因表达变化。Objective To study the effect of pre-degenerated common peroneal nerve on apoptosis and proliferation in injured rat optic nerve. Methods One hundred and fifteen adult male rats were divided into normal, injured and transplantation group. Cell number, apoptosis/proliferation, proliferating cell nuclear antigen （ PCNA ） expression and apoptosis/proliferation related gene expression in rat optic nerves were examined respectively using HE staining, flow cytometry, Western blotting, gene chips and real-time PCR. Results Cells in the optic nerve of the injury group increased compared with that in the normal group, but was lower than that in the transplantation group. Compared with the normal group, apoptotic cells increased and proliferated ceils decreased in the injury group; Compared with the injury group, apoptotic cells decreased and proliferated ceils increased in the transplantation group. Compared with the normal group, PCNA expression was down-regulated in the injury group, but PCNA expression was up-regulated in the transplantation group than that in the injury group. Compared with normal group, thirty seven apoptosis and proliferation related genes expressed differentially in the injury group; Compared with the injury group, three genes expressed differentially in the transplantation group. Conclusion The cellular apoptosis increases and the cellular proliferation decreases in the distal part of the optic nerve after the optic nerve injury. Autograft transplantation of the common peroneal nerve could reduce the cellular apoptosis and promote the cellular proliferation. In addition, some corresponding gene expressions changed.

关 键 词：视神经 移植 增殖 流式细胞仪 基因芯片 荧光定量PCR 免疫印迹法 大鼠 

分 类 号：R774.6[医药卫生—眼科]