EZH2在肝细胞癌中的表达及临床意义  

Expression and clinical significance of EZH2 in hepatocellular carcinoma

作　　者：陆海霞[1] 危丹明[1] 冯震博[1] 

机构地区：[1]广西医科大学第一附属医院病理科,南宁530021

出　　处：《解剖学报》2011年第1期75-79,共5页Acta Anatomica Sinica

基　　金：广西科学基金资助项目(0728153)

摘　　要：目的探讨EZH2在肝细胞癌(肝癌)中的表达及其临床意义。方法采用RT-PCR、免疫组织化学和Western blotting方法分析肝癌及癌旁组织EZH2mRNA转录和蛋白表达水平及其与临床病理因素的关系。结果在42例肝癌组织中检出26例EZH2mRNA,而在相应的42例癌旁组织中只检出12例,癌组织EZH2mRNA阳性率高于癌旁组织,差异有显著性(P<0.01)。免疫组织化学结果显示,42例肝癌组织中有25例呈EZH2蛋白表达阳性,相应的42例癌旁组织中只有9例呈阳性,癌组织和癌旁组织蛋白表达率有显著性差异(P<0.01)。取上述42例中的18例肝癌及癌旁组织做Western blotting,结果显示,18例肝癌及癌旁组织均有EZH2蛋白表达,癌组织EZH2表达量明显高于癌旁组织(0.90±0.30和0.36±0.21,P<0.01)。EZH2与临床病理特征分析中,EZH2mRNA在低分化组的表达明显高于高-中分化组,而与肿瘤直径、肝内外转移无相关性。但EZH2蛋白表达与肿瘤直径、分化程度、肝内外转移之间均无相关性。结论 EZH2在肝癌组织中表达上调,提示EZH2在肝癌的发生发展中起重要作用。Objective To study the expression of EZH2 gene in hepatocellular carcinoma （HCC） and its clinical roles. Methods Reverse transcription-polymerase chain reaction （RT-PCR）, immunohistoehemistry, and Western blotting were used to detect the EZH2 mRNA and EZH2 protein expression in the tumor samples and surrounding non- cancerous liver samples, and then investigated the correlation between expression status and various elinicopathological parameters. Results The positive rate of EZH2 mRNA in HCC was 61.9% （26/42） ,the EZH2 mRNA positive rate in surrounding non-cancerous liver samples was 28.6% （12/42）. EZH2 mRNA expression in HCC was significantly higher than that in surrounding non-cancerous liver samples （P 〈 0.01 ）. Immunohistochemistry showed EZH2 protein expression in HCC was also significantly higher than that in surrounding non-cancerous liver samples （25/42 vs 9/42, P 〈 O. 01 ）. Western blotting showed that EZH2 protein was expressed in all selected 18 HCC and surrounding non-cancerous liver cases. The quantity of EZH2 protein in HCC was much more than that in surrounding non-cancerous tissue（0. 90 ＋ 0.30 vs 0. 36 ~ 0. 21 ,P 〈 0. 01 ）. As the EZH2 and pathologic parameters were analysed, it was found that EZH2 mRNA transcript was elevated in poorly differentiated HCC compared with well-moderately differentiated HCC,but there were no statistically significant correlations between EZH2 mRNA and tumor size, and iutrahcpatic/ extrahepatic metastasis. No significant difference was found between EZH2 protein and tumor size, differentiation, and intrahepatic/ extrahepatic metastasis. Conclusion The expression of EZH2 gene was up-regulated in HCC, which might play an important role in oncogenesis and development of HCC.

关 键 词：肝细胞癌 EZH2基因 反转录一聚合酶链反应 免疫组织化学 免疫印迹法 人 

分 类 号：R735.7[医药卫生—肿瘤]