含蟾酥胶囊血清诱导人肝癌BEL-7402细胞凋亡并下调Bcl-2的表达  

Chansu capsule with medicated serum induces apoptosis and downregulates Bcl-2 protein expression in BEL-7402 cells

作　　者：顿爱社[1] 张春菊[2] 吕伯实[1] 刘帅[1] 侯海峰[3] 王凡[4] 

机构地区：[1]泰山医学院解剖学教研室,山东泰安271016 [2]泰安卫生学校基础护理教研室,山东泰安271016 [3]泰山医学院流行病学研究所,山东泰安271016 [4]四川大学华西基础与法医学院,四川成都610041

出　　处：《解剖科学进展》2011年第1期54-59,共6页Progress of Anatomical Sciences

基　　金：泰山医学院青年基金项目(No.2007ZRQN033)

摘　　要：目的探讨含蟾酥胶囊血清诱导人肝癌BEL-7402细胞凋亡的作用机制。方法用中药血清药理学方法,不同浓度含药血清加入体外培养的人肝癌BEL-7402细胞,分别孵育24h、48h,显微镜下观察细胞形态学变化,MTT比色法检测细胞存活率,流式细胞仪检测细胞凋亡率,琼脂糖凝胶电泳测定DNA梯状条带,免疫细胞化学染色检测Bcl-2蛋白的表达。结果含蟾酥胶囊血清组部分细胞出现凋亡细胞形态学改变,细胞存活率降低,增殖受到抑制;流式细胞仪检测,可见明显的凋亡峰;孵育48h,琼酯糖凝胶电泳呈现梯状条带(DNALadder);免疫细胞化学检测显示Bcl-2表达明显降低。结论蟾酥胶囊可以诱导人肝癌细胞BEL-7402凋亡,其作用机制之一可能与下调Bcl-2蛋白的表达有关。Objective To investigate the efficiency of Chansu capsule with medicated serum（CCMS） in inducing the apoptosis of human hepatocellular carcinoma（HCC） BEL-7402 cells. Methods Using serum pharmacologic method of the traditional chinese medicine,we chose different concentrations of CCMS（10%, 20%, 30%） to co-cultivate with HCC BEL-7402 cells for 24 or 48 hours.The morphological change of apoptotic HCC cells was observed with light microscope, the cell growth was examined by methyl-tetrazolium （MTT） assay, the percentage of apoptotic cells was examined by flow cytometry, DNA ladder was examined by agar gel electrophoresis, the expression of Bcl-2 protein was measured with immunocytochemistric staining. Results Some cells of CCMS groups were seen to be smaller and rounder with single floating growth, the cell growth was remarkably inhibited by the medicated serum contaning chansu capsule, the apoptotic cell peaks were observed by flow cytomeric assays, DNA Ladder appeared by co-culturing for 48 hours, the expression level of Bcl-2 protein decreased compared with control. Conclusion CCMS suppresses the growth of BEL-7402 cells by inducing apoptosis ,which may be related to the downregulation of Bcl-2 protein.

关 键 词：蟾酥胶囊血清 BEL-7402细胞 凋亡 BCL-2 

分 类 号：R735.7[医药卫生—肿瘤]