精子蛋白32/OY-TES-1在视网膜母细胞瘤中的表达  

Expression of sperm protein 32/OY-TES-1 in retinoblastoma

作　　者：孔旻[1] 王玲[1] 王树先[1] 谢小薰[2] 梁皓[1] 

机构地区：[1]广西医科大学第一附属医院眼科中心,南宁530021 [2]广西医科大学组织胚胎学教研室

出　　处：《中华眼底病杂志》2011年第1期17-20,共4页Chinese Journal of Ocular Fundus Diseases

基　　金：国家自然科学基金（30760055）;广西科技厅青年基金（桂科青 0542052）;广西卫生厅科研经费资助项目（桂卫Z2006049）;广西高发疾病研究创新性团队基金（2007-59）

摘　　要：目的 观察精子蛋白（SP）32/OY-TES-1在视网膜母细胞瘤（RB）组织中的表达.方法 病理科收检并经病理学检查确诊的30例RB患儿的石蜡标本纳入研究.采用逆转录-聚合酶链反应（RT-PCR）技术检测15例RB组织、12例非肿瘤病变视网膜组织及22例眼部正常组织中SP32/OY-TES-1mRNA的表达;免疫组织化学技术对30例RB组织和24例正常视网膜组织中SP32/OY-TES-1蛋白的表达进行检测,对比分析SP32/OY-TES-1 mRNA和蛋白表达与RB患儿年龄、性别、肿瘤大小、肿瘤分化程度和临床分期的相互关系.结果 RB组织、非肿瘤病变视网膜组织、眼部正常组织SP32/OY-TES-1mRNA的表达率分别为86.7%、16.7%和36.4%.不同性别（χ^2=0.744）、年龄≤2岁和＞2岁（χ^2=1.178）、临床分期（χ^2=1.188）、肿瘤大小（χ^2=0.216）、肿瘤分型（χ^2=1.885）之间SP32/OY-TES-1基因阳性表达率比较,差异无统计学意义（P＞0.05）.SP32/OY-TES-1蛋白在RB中的表达率为73.3%,在正常视网膜组织中则不表达.不同性别（χ^2=1.000）、年龄≤2岁和＞2岁（χ2=0.403）、不同肿瘤大小（χ^2=2.274）、不同肿瘤分型（χ^2=0.138）之间SP32/OY-TES-1蛋白阳性表达率比较,差异无统计学意义（P＞0.05）;不同临床分期（χ^2=6.193）、视神经是否浸润（χ^2=4.535）之间SP32/OY-TES-1蛋白阳性表达率比较,差异有统计学意义（P＜0.05）.结论 SP32/OY-TES-1高表达于RB组织中;SP32/OY-TES-1蛋白在RB组织中的表达率与视神经浸润和临床分期存在密切的关系.Objective To observe the expression of sperm protein （SP） 32/OY-TES-1 in retinoblastoma （RB）. Methods Thirty paraffin specimens of pathologically confirmed RB eyeballs were investigated in this study. The SP32/OY-TES-1 mRNA expression of 15 RB tissues, 12 non-tumor retinal tissues and 22 normal eye tissues were detected by reverse transcription polymerase chain reaction （RT-PCR）. The SP32/OY-TES-1 protein expression of 30 RB tissues and 24 normal retinal tissues were examined by immunohistochemistry. The relationship between SP32/OY-TES-1 mRNA, protein expression and age, gender, tumor size, tumor differentiation, clinical stage of RB children were analyzed. Results The expression rate of SP32/OY-TES-l mRNA in RB tissues, non-tumor retinal tissues and normal eye tissues were 86.7%, 16. 7% and 36. 4% respectively. Compared the expression rate of SP32/OY-TES-1mRNA in different gender （χ^2 = 0.744 ）, age （χ^2 = 1.178 ）, clinical stage （χ^2 = 1. 188 ）, tumor size （χ^2 =0. 216） ,tumor differentiation （χ^2 = 1. 885）,the differences were not statistically significant （P〉0. 05）. The expression rate of SP32/OY-TES-1 protein in RB tissues was 73.3% and no expression in normal retinal tissues. Compared the expression rate of SP32/OY-TES-1 protein in different gender （χ^2= 1.000）, age with ≤ two years and 〉 two years （χ^2 = 0. 403）, tumor size （χ^2 = 2. 274）, tumor differentiation （χ^2=0. 138）, the differences were not statistically significant （P〉0.05） ;but in clinical stage （χ^2 =6. 193） ,with or without optic nerve infiltration （χ^2= 4. 535）, the differences were statistically significant （P〈0.05）.Conclusions SP32/OY-TES-1 is highly expressed in RB. The expression rate of SP32/OY-TES-1 is related to optic nerve infiltration and clinical stage of RB.

关 键 词：视网膜母细胞瘤/病理生理学 基因表达 抗原 肿瘤 

分 类 号：R739.7[医药卫生—肿瘤]