survivin和GRIM-19在前列腺癌组织中的表达  

Expressions of survivin and GRIM-19 in prostate cancer

作　　者：刘艳波[1] 沈维高[1] 葛贺[1] 盖晓东[1] 芦丽莉[1] 赵雪俭[2] 

机构地区：[1]北华大学基础医学院病理生理学教研室,吉林吉林132013 [2]吉林大学前列腺疾病防治研究中心,吉林长春132001

出　　处：《中华男科学杂志》2011年第1期21-26,共6页National Journal of Andrology

基　　金：国际科技合作重点项目(2004DFB02000)~~

摘　　要：目的:探讨survivin和GRIM-19在前列腺癌组织中的表达及意义。方法:应用免疫组化、RT-PCR和Western印迹法检测survivin和GRIM-19在正常前列腺(NP)组织、良性前列腺增生(BPH)组织和前列腺癌(PCa)组织中的表达情况。结果:免疫组化结果显示survivin在NP、BPH和PCa组织中的表达率分别为6.25%、18.18%和90.62%(P<0.01);GRIM-19的表达率分别为87.50%、81.82%和9.37%(P<0.01)。半定量RT-PCR结果显示,在NP和BPH组织未检测到survivinmRNA表达,而PCa组织可检测到survivinmRNA高表达。Western印迹结果证实,在NP和BPH组织中有微量的survivin蛋白表达;而PCa组织可检测到survivin蛋白高表达;在NP和BPH组织中GRIM-19蛋白表达强阳性,而PCa组织中只有微量表达,两者比较差异均有显著性(P<0.01)。结论:survivin和GRIM-19的表达可能与PCa的发生密切相关。Objective：To investigate the expressions of survivin and GRIM-19 in prostatic cancer tissue and their clinical implications.Methods：We detected the expressions of survivin and GRIM-19 in the tissues of normal prostate（NP）,benign prostate hyperplasia（BPH） and prostate cancer（PCa） using immunohistochemical staining,RT-PCR and Western blot,and processed the data by SPSS12.Results：The positive rates of survivin expression were 6.25%,18.18% and 90.62% in NP,BPH and PCa（P 0.01）,while those of GRIM-19 were 87.50%,81.82% and 9.37%,respectively（P 0.01）.Semiquantitative RT-PCR and immunohistochemical staining showed that both survivin mRNA and survivin expressions were highly positive in PCa but negative in NP and BPH.Western blot exhibited that the survivin protein was expressed strongly in PCa but weakly in NP and BPH,while the GRIM-19 protein was expressed just contrariwise（P 0.01）.Conclusion：The expressions of survivin and GRIM-19 may be closely correlated with the pathogenesis of prostate cancer.

关 键 词：SURVIVIN GRIM-19 前列腺癌 免疫组化 WESTERN印迹 

分 类 号：R737.25[医药卫生—肿瘤]