乌梢蛇精巢间质细胞微细结构与血清睾酮浓度的相关性  

The Correlation between Fine Structure of Leydig Cells and Serum Testosterone in Chinese Rat Snake Zaocys dhumnades

作　　者：常小娟[1] 梁刚[1] 王琼霞[1,2] 

机构地区：[1]陕西师范大学生命科学学院教育部药用资源与天然药物化学重点实验室西北濒危药材资源开发国家工程实验室,西安710062 [2]北京苹果园中学

出　　处：《动物学杂志》2011年第1期29-35,共7页Chinese Journal of Zoology

摘　　要：用光镜及透射电镜观察了乌梢蛇(Zaocys dhumnades)精巢间质细胞的显微与超微结构,并利用放射免疫测定法测定了血清中睾酮浓度。结果表明,在一个年生殖周期中,乌梢蛇间质组织所占区域相对大小、间质细胞数量和显微结构均存在较明显的变化;5月份的间质细胞具有发达的管状嵴线粒体、丰富的滑面内质网、大量的脂滴等合成和分泌类固醇激素的超微结构特征;血清中睾酮浓度变化明显,5月份出现主峰,9月份出现次峰。间质细胞的显微和超微结构特征及其变化与血清中睾酮浓度变化具有相关性,表明乌梢蛇间质细胞能够合成与分泌睾酮等性类固醇激素。The microstructure and ultrastructure of Leydig cells in Chinese Rat Snake Zaocys dhumnade were examined by light microscopy and transmission electron microscopy, and the concentration of serum testosterone was measured by radioimmunoassay （RIA）. During a reproductive cycle, the region of Leydig tissue, the quantity of Leydig cells and the mierostrueture changed very evidently. In the Leydig cells in May, there were developed mitochondria with tubular cristae, abundant smooth endoplasmic reticulum and numerous lipid droplets,suggesting that Leydig cells have the ultrastructure characteristic of synthesizing and secreting steroid hormone. The concentration of serum testosterone also changed evidently, and its major peak appeared in May, and the minor peak appeared in September. The microstructure and ultrastructure of Leydig cells were positively correlated with the concentration of serum testosterone. Our results suggest that Leydig cells in Chinese Rat Snake can synthesize and secrete testosterone.

关 键 词：乌梢蛇 精巢 间质细胞 微细结构 睾酮 

分 类 号：Q952.4[生物学—动物学] Q954