IFN-γ联合全反式维A酸对人皮肤鳞状细胞癌SCL-1细胞生长凋亡的影响  

Effect of interferon-γand all-trans retinoic acid （ATRA） on the proliferation of and apoptosis in a human cutaneous squamous cell carcinoma cell line SCL-1

作　　者：王珍[1] 刘梅[2] 李铁男[1] 朱红[2] 王雅坤[2] 何春涤[2] 

机构地区：[1]沈阳市第七人民医院,110003 [2]中国医科大学附属第一医院皮肤科,沈阳110001

出　　处：《中华皮肤科杂志》2011年第1期35-38,共4页Chinese Journal of Dermatology

基　　金：教育部高等学校博士学科点专项科研基金（20092104110003）;辽宁省教育厅创新团队项目计划（2008T193,2006T133）;辽宁省高等学校优秀人才支持计划（LR201040）;沈阳市卫生局科研课题计划项目（2008-52）

摘　　要：目的 探讨IFN-γ联合全反式维A酸（ATRA）在体外对人皮肤鳞状细胞癌SCL-1细胞形态分化、增殖及生长凋亡的影响.方法 SCL-1细胞根据处理因素不同分为,①对照组;②1μmol/L ATRA组;③100 U/ml IFN-γ组;④500 U/ml IFN-γ组;⑤1000 U/ml IFN-γ组;⑥1000 U/ml IFN-γ＋1μmol/LATRA组.用噻唑蓝（MTT）和流式细胞仪（FCM）分析细胞增殖及凋亡的变化.透射电镜、1%碘化丙啶染色及倒置相差显微镜观察细胞凋亡形态.结果 细胞的增殖抑制有明显IFN-γ浓度依赖性,其中1000 U/ml浓度抑制作用最明显.ATRA及IFN-γ能诱导SCL-1细胞凋亡,凋亡率分别是4.84%和11.96%,二者联合诱导凋亡率为18.71%;透射电镜及1%碘化丙啶染色可见1 μmol/L ATRA和1000 U/ml IFN-γ处理的SCL-1细胞发生一系列凋亡特征性的形态学改变,并见较多早期凋亡细胞.IFN-γ及ATRA诱导SCL-1细胞形态呈双极性改变,趋向良性分化.结论 IFN-γ可促进SCL-1细胞分化,抑制其增殖,在一定范围内有剂量依赖性,且与ATRA具有协同作用.Objective To investigate the in vitro effect of interferon-γ （IFN-γ） and ATRA on the morphological transition, proliferation of and apoptosis in a human cutaneous squamous cell carcinoma cell line SCL-1. Methods Cultured SCL-1 cells were divided into 6 groups to be treated with ATRA of 1 μmol/L, various concentrations （ 100, 500, 1000 U/ml） of IFN-γ, the combination of ATRA of 1 μmol/L and IFN-y of 1000 U/ml,respectively, or to remain untreated. MTT assay and flow cytometry were performed to evaluate the cell proliferation and apoptosis. The morphological features of apoptotic cells were observed by a transmission electron microscope （TEM） and inverted phase contrast microscope after 1% propidium iodide staining. Results IFN-γ could inhibit the proliferation of SCL-1 cells in a dose-dependent manner, and the most pronounced inhibitory effect was observed at a dose of 1000 U/ml . ATRA and IFN-γ induced an apoptosis in SCL-1 cells, and the early apoptosis rate was 4.84%, 11.96% and 18.71% in SCL-1 cells after treated with ATRA of 1 μmol/L, IFN-γ of 1000 U/ml and their combination, respectively. A series of morphological changes characteristic of apoptosis,such as bipolar changes, were observed in SCL-1 cells treated with ATRA and IFN-γ, with the presence of many early apoptotic cells, which showed a trend towards benign differentiation. Conclusions Within a certain concentration range, IFN-γcan promote the differentiation, but inhibit the proliferation of SCL-1 cells in a dose-dependent manner, and ATRA could enhance the effects of IFN-γ.

关 键 词：干扰素Ⅱ型 维甲酸 肿瘤细胞 培养的 细胞凋亡 肿瘤 鳞状细胞 

分 类 号：R739.5[医药卫生—肿瘤]