葡萄糖转运蛋白-3及缺氧诱导因子1α在星形细胞瘤中的表达  

Expressions of glucose transporter-3 and hypoxia-inducible factor-1α in astrocytoma

作　　者：刘阳[1] 杨福兵[2] 刁正文[2] 刘卫平[3] 费舟[3] 章翔[3] 

机构地区：[1]绵阳市第三人民医院神经外科,621000 [2]泸州医学院附属医院神经外科,泸州646000 [3]第四军医大学西京医院神经外科,西安710032

出　　处：《中华神经医学杂志》2011年第1期29-32,共4页Chinese Journal of Neuromedicine

摘　　要：目的 探讨葡萄糖转运蛋白-3（GLUT-3）和缺氧诱导因子（HIF）-1α在星形细胞瘤中的表达及其临床意义.方法 应用免疫组化染色和RT-PCR方法检测80例星形细胞瘤患者肿瘤组织中GLUT-3和HIF-1α表达情况,另取10例星形细胞瘤周围做内减压切除的正常脑组织为对照.结果 GLUT-3在Ⅰ、Ⅱ、Ⅲ、Ⅳ级星形细胞瘤中阳性表达率分别为35%、60%、80%、100%,HIF-1α在Ⅰ、Ⅱ、Ⅲ、Ⅳ级星形细胞瘤中阳性表达率分别为20%、50%、75%、95%,两者呈正相关关系（r=0.550,P=0.000）.GLUT-3 mRNA表达在Ⅰ、Ⅱ、Ⅲ、Ⅳ级星形细胞瘤中分别为0.19±0.03、0.45±0.12、0.67±0.11、0.89±0.19,HIF-1α mRNA在Ⅰ、Ⅱ、Ⅲ、Ⅳ级星形细胞瘤中分别为0.21±0.06、0.48±0.11、0.72±0.16、0.93±0.12,各级别间比较差异均有统计学意义（P=0.000）,且GLUT-3 mRNA、HIF-1αmRNA表达强度与星形细胞瘤的病理分级呈正相关关系（r=0.887,P=0.000;r=0.813,P=0.000）,二者之间也呈正相关关系（r=0.530,P＜0.05）.结论 GLUT-3与HIF-1α表达强度和星形细胞瘤的病理分级成正相关,可以作为判断星形细胞瘤生物学行为的重要指标.Objective To explore the expressions and clinical significances of glucose transporter protein-3 （GLUT-3） and hypoxia-inducible factor-1α （HIF-1α） in astrocytoma. Methods The astrocytoma tissues from 80 patients with astrocytoma were chosen as experimental group; and the normal cerebral tissues from another 10 patients performed depression around the astrocytoma were chosen as control group. Immunohistochemistry and RT-PCR were employed to detect the GLUT-3 and HIF-1α expressions in these tissues. Results The protein of GLUT-3 in grade Ⅰ, grade Ⅱ, grade Ⅲ and grade Ⅳ astrocytomas were positively expressed, respectively, as 35%, 60%, 80% and 100% by immunohistochemistry; the protein of HIF-1α in grade Ⅰ, grade Ⅱ, grade Ⅲ and grade Ⅳ astrocytomas were positively expressed, respectively, as 20%, 50%, 75% and 95% by immunohistochemistry; positive correlation was noted between the protein levels of GLUT-3 and HIF-1α （r=0.550, P=0.000）. The mRNA levels of GLUT-3 in grade Ⅰ, grade Ⅱ, grade Ⅲ and grade Ⅳ astrocytomas were expressed, respectively,as 0.19±0.03, 0.45±0.12, 0.67±0.11 and 0.89±0.19 by RT-PCR; the mRNA levels of HIF-1α were expressed, respectively, as 0.21 ±0.06, 0.48±0.11, 0.72±0.16 and 0.93±0.12 by RT-PCR; and the mRNA expression level of GLUT-3 were positively correlated to that of HIF-1α （r=0.530, P=0.000）.Significant difference between each 2 different pathological grades of astrocytoma was noted （P〈0.05）. The mRNA expression levels of GLUT-3 and HIF-1α were positively correlated to the pathological grades of astrocytomas （r=0.887, P=0.000; r=0.813, P=0.000）. Conclusion The expression of GLUT-3 and HIF-1α in astrocytoma are significantly correlated to tumors＇ pathology grade, which can be taken as a useful marker for predicting the biological behavior.

关 键 词：神经胶质瘤 葡萄糖转运蛋白-3 缺氧诱导因子-1Α 

分 类 号：R739.4[医药卫生—肿瘤]