在小鼠帕金森病模型中观察丙戊酸钠对多巴胺神经元及GDNF表达的影响(英文)  

Effects of valproate on dopaminergic neurons and expressions of GDNF in Parkinson's disease mice

作　　者：张玉梅[1] 吴雪飞[2] 于德钦[2] 彭岩[2] 张万琴[2] 赵杰[2] 

机构地区：[1]辽宁中医药大学附属医院中医内科,沈阳110032 [2]大连医科大学生理学教研室,大连116044

出　　处：《神经解剖学杂志》2011年第1期1-7,共7页Chinese Journal of Neuroanatomy

基　　金：National Natural Science Funds of China(No.30770737)~~

摘　　要：目的:通过建立小鼠帕金森病模型,观察丙戊酸钠(valproate,VPA)对中脑黑质多巴胺神经元及胶质源性神经营养因子(glial-derived neurotrophic factor,GDNF)表达的影响,以期探索丙戊酸钠的神经保护作用机制。方法:采用免疫组织化学方法检测小鼠中脑黑质多巴胺神经元;原位杂交方法检测纹状体、海马、顶叶皮质GDNF的表达。结果:GDNF在模型组和VPA组表达明显增加。结论:VPA可能通过上调GDNF的表达而起到神经保护作用。Objective：To investigate the expression of glial-derived neurotrophic factor（GDNF） in mice with Parkinson＇s disease,and to study the neuroprotective effects of valproate（VPA）.Methods：The animals were randomly divided into four groups,including normal saline（NS）＋NS group,NS＋MPTP group,VPA ＋MPTP group and VPA＋NS group,with 6 mice in each group.Tyrosine hydroxylase（TH） in the substantia nigra pars compacta（SNc） was measured by immunohistochemistry.The expression of GDNF in striatum,hippocampus and cerebral cortex was observed by in situ hybridization.The A values of positive neurons of GDNF was scaned and analyzed statistically.Results：The expression of GDNF was significantly increased in normal saline（NS）＋MPTP group,VPA ＋MPTP group and VPA＋NS group compared to NS＋NS group.Conclusion：Protective effect of VPA against Parkinson＇s disease may be associated with increased expression of GDNF.

关 键 词：帕金森病 胶质源性神经营养因子 丙戊酸钠 小鼠 

分 类 号：R742.5[医药卫生—神经病学与精神病学]