坐骨神经分支选择性损伤后降钙素相关肽在大鼠中央杏仁核中的表达变化  

Changes of calcitonin gene-related peptide expression in central nucleus of amygdala in rat model of spared nerve injury

作　　者：徐飞[1] 赵宁[2] 汤雨[3] 千年松[4] 赵力[5] 廖永晖[6] 杨雁灵[6] 王亚云[7] 

机构地区：[1]解放军第302医院肿瘤放射治疗中心,北京100039 [2]空军总医院医疗科,北京10036 [3]解放军第302医院医学影像中心,北京100039 [4]解放军总医院肝胆外科,北京100853 [5]海军总医院心内科,北京100048 [6]第四军医大学西京医院肝胆外科,西安710032 [7]第四军医大学人体解剖与组织胚胎学教研室暨梁銶琚脑研究中心,西安710032

出　　处：《神经解剖学杂志》2011年第1期14-18,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(30971174;81071895;81070327)

摘　　要：目的:观察降钙素基因相关肽(calcitonin gene-related peptide,CGRP)在坐骨神经分支选择性损伤(spared nerve injury,SNI)模型大鼠中央杏仁核中的表达变化。方法:健康SD大鼠分为假手术对照组(n=60)和坐骨神经分支选择性损伤组(n=60),实验组损伤后饲养3 d,7 d,14 d,免疫荧光染色结合图像分析技术观察CGRP在中央杏仁核中的表达变化。结果:CGRP分布于正常SD大鼠中央杏仁核,SNI后3 d,损伤组大鼠中央杏仁核中的CGRP表达开始升高,图像分析表明CGRP的光密度与对照组比较,差异有统计学意义(P<0.01);SNI后14 d CGRP表达达最高峰(P<0.01)。结论:SNI后CGRP可能通过杏仁核参与神经病理性疼痛的调控。Objective：To study the changes of calcitonin gene-related peptide（CGRP） expression in central nucleus amygdale in rat model of spared nerve injury.Methods：Healthy Sprague Dawley rats were randomly divided into sham operation group（n=60） and experimental group（n=60） which were killed at day 3,7 and 14 after spared nerve injury.The distribution and quantity of CGRP in central nucleus of amygdala was analyzed by immunofluorescence and image analysis methods.Results：CGRP-immunoreactivity（IR） existed in the central nucleuses of amygdale in normal Sprague Dawley rats.An initial increase of CGRP-IR was noted and the content of CGRP under image analysis was significantly higher compared with that of control group（P0.01） at day 3 after undergoing spared nerve injury and rise to a maximum level at day 14（P0.01）.Conclusion：It was indicated that CGRP might play a role in the regulation of neuropathic pain after spared nerve injury.

关 键 词：坐骨神经 降钙素基因相关肽 中央杏仁核 神经病理性痛 大鼠 

分 类 号：R741[医药卫生—神经病学与精神病学]