树鼩海马微环境Ca^(2+)异常与rCBF相互作用机制的探讨  

Explore the mutual action mechanism between the Ca^(2+) abnormality in the microenvironment of hippocampusand regional cerebral blood flow in Tree Shrews

作　　者：罗海芸[1] 罗耀辉[2] 李树清[3] 

机构地区：[1]昆明医学院药理学教研室,昆明650500 [2]昆明医学院生理学教研室,昆明650500 [3]昆明医学院病理生理学教研室,昆明650500

出　　处：《神经解剖学杂志》2011年第1期63-66,共4页Chinese Journal of Neuroanatomy

基　　金：云南省自然科学基金(2006c0036Q)

摘　　要：目的:探讨海马微环境内异常Ca2+与局部脑血流(regional cerebral blood flow,rCBF)相互作用变化及其可能机制。方法:单泵等速微灌流系统分别行树鼩海马高、中、低Ca2+微灌流4 h,通过激光多普勒血流计测量海马CA1区rCBF含量;并用电镜观测海马血管内皮细胞的超微结构。微灌流Ca2+溶液后原位灌流尼莫地平,观测上述指标的变化。结果:树鼩微环境内随着Ca2+的增加海马rCBF逐渐降低(P<0.01),其中高浓度Ca2+微灌流后的rCBF(PU)最低(2.9±0.81,P<0.01),使用Ca2+通道拮抗剂尼莫地平后可显著升高rCBF,同时在电镜下可观察到血管内皮细胞的损伤有所减轻。结论:缺血时rCBF的降低会引起微环境内Ca2+降低,致使微环境异常;而微环境内Ca2+异常也可致rCBF降低。Objective：To reveal the mutual action and possible mechanism of abnormality of calcium ion in the microenvironment of hippocampus and regional cerebral blood flow（rCBF）.Methods：A lateral hippocampus was microperfused by individual high,middle,low concentration of calcium ion during 4 h by a kind of single-pumped push-pull perfusion system in Tree Shrews.The rCBF in hippocampus CA1 area was detected by laser-Doppler（LD） flow meter and the ultramicrostructure of blood vessel endothelium in hippocampus was observed.Then the change of above-mentioned index are observed when perfused Nimodipine in situ.Results：The rCBF gradually reduced along with heighten concentration of calcium ion（P0.01）,especially in high concentration（2.9±0.81,P0.01）;But the rCBF significant increased after utilization the calcium ion channal antagonist Nimodipine,and that the damage of vascular endothelial cells was also observed the decrease under electron microscope.Conclusion：The decrease of rCBF can degrade calcium ion in the microenvironment when ischemia happen,and induce abnormal microenvironment.The abnormality of calcium ion in the microenvironment of hippocampus also causes the decrease of rCBF.

关 键 词：脑缺血 微环境 CA2+ 局部脑血流 树鼩 

分 类 号：R743[医药卫生—神经病学与精神病学]