Pygo2在脑胶质瘤组织和细胞的表达及临床意义  

Expression and Clinical Significance of Pygo2 in Glioma Cells and Tissue

作　　者：王海东[1] 陈玉英[2] 王占祥[1] 刘希尧[1] 谭国伟[1] 沈上杭[1] 

机构地区：[1]厦门大学附属第一医院神经外科,福建省厦门市361003 [2]重庆邮电大学生物信息学院

出　　处：《中国肿瘤临床》2011年第2期75-78,共4页Chinese Journal of Clinical Oncology

基　　金：福建省自然科学基金(编号:2009D002);厦门市科技局基金(编号:3502z20089001);中国博士后基金(编号:20080440728);重庆市教委科技项目基金资助(编号:KJ100504)~~

摘　　要：目的:检测Pygo2在脑胶质瘤组织和细胞中的表达,并探讨其临床意义。方法:采用RT-PCR检测5种胶质瘤细胞株Pygo2 mRNA表达,Real-time PCR检测67例Ⅱ～Ⅳ级脑胶质瘤组织中Pygo2 mRNA表达,免疫组织化学SP法检测80例脑胶质瘤组织中Pygo2的蛋白表达。结果:Pygo2 mRNA在5种胶质瘤细胞株中均有表达,其中U251细胞中最高,C6细胞中最低。Pygo2mRNA在正常脑组织中表达极低,而在脑胶质瘤Ⅱ～Ⅳ级表达显著增高,且Pygo2 mRNA表达随肿瘤分级的增高而显著增强(P<0.05)。Pygo2在脑胶质瘤组织细胞中定位于胞核,在正常脑组织中表达呈阴性,而在脑胶质瘤组织中约有65.0%阳性表达,两者差异显著(P<0.01)。Pygo2表达Ⅱ级(WHOⅡ)中低,Ⅳ级(WHOⅣ)中高。Pygo2在脑胶质瘤Ⅰ级、Ⅱ级、Ⅲ级、Ⅳ级中表达阳性率分别是5/13(38.5%)、12/26(46.2%)、15/19(78.9%)、20/22(90.9%),Pygo2蛋白表达与肿瘤分级密切相关(P<0.01)。此外,Pygo2蛋白表达水平与患者的性别、年龄均无显著性差异(P>0.05)。结论:Pygo2在正常脑组织中呈阴性,在脑胶质瘤组织和细胞中均高表达,且在组织中其表达随胶质瘤级别增高而显著增强,提示Pygo2可能在脑胶质瘤的发生、恶性演进中起了重要作用。Objective： To detect mRNA and protein expression of Pygopus2 （Pygo2） in glioma tissues and cells, and to study the clinical significance. Methods： The mRNA expression of Pygo2 in 5 glioma cell lines was quantified by reverse transcription-polymerase chain reaction （ RT-PCR ）, and mRNA expression of Pygo2 in 67 II-IV glioma tissue samples was quantified by Real-time Reverse Transcription （ RT ）-PCR analysis, and protein expression of Pygo2 was evaluated in 80 of glioma tissue samples by SP Immunohistochemistry （ IHC ） analysis. Results： Pygo2 mRNA was expressed in all of five cell lines with the highest expression in U251 cells and the lowest expression in C6 cells. Pygo2 mRNA expression was very low in normal brain specimens, and the mRNA expression of Pygo2 was significantly increased in II -IV glioma grade compared with that in normal brain samples. Moreover, the increased Pygo2 mRNA expression was correlated with the high grade of human glioma （ P 〈 0.05 ）. Pygo2 protein was locatated in the nucleus of glioma epithelial cells. Negative immunostaining for Pygo2 protein was observed primarily in normal brain tissue sections, and positive immunostaining for Pygo2 protein was detected in 52/80 （ 65.0 % ） glioma samples, with significant difference between normal brain tissue and glioma samples （ P 〈 0.01 ）. Weak and strong immunostaining was shown in low malignant （ WHO II ） and highly malignant （ WHO IV ） brain gliomas respectively. The frequency of positive expression for individual pathological stages was 5/13 （ 38.5 % ） for stage I, 12/26 （ 46.2 % ） for stage II, 15/19 （ 7 8.9 % ） for stage III, and 20/22 （ 90.9% ） for stage IV. Pygo2 protein expression was significantly correlated with tumor grade （ P 〈 0.01 ）. In addition, Pygo2 protein expression was not related to patients＇ gender or age （ P 〉 0.05 ）. Conclusion： Pygo2 protein expression was not tound in normal brain tissue. Pygo2 was highly expressed in glioma tissue, and was co

关 键 词：Pygopus2 REAL-TIME PCR脑胶质瘤表达 

分 类 号：R739.4[医药卫生—肿瘤]