血管内皮生长因子B及其变位剪切体在不同肝组织中的表达差异及意义  

Significance of expression of vascular endothelial growth factor B and its splice isoforms in different types of liver lesions

作　　者：王瞿辉[1] 王毅军[2] 高英堂[3] 杜智[3] 杨斌[4] 张晔[3] 郭华[5] 景丽[5] 余俊[3] 许彦杰[3] 

机构地区：[1]天津医科大学第三中心临床学院,天津市300170 [2]天津市第三中心医院肝胆外科,天津市300170 [3]天津市第三中心医院天津市人工细胞重点实验室,天津市300170 [4]南开大学生命科学学院,天津市300071 [5]天津市第三中心医院天津市肝胆疾病研究所,天津市300170

出　　处：《世界华人消化杂志》2011年第1期31-37,共7页World Chinese Journal of Digestology

基　　金：天津市重点科技攻关专项基金资助项目;No.05YFSJSF02500~~

摘　　要：目的:探讨不同肝组织中VEGF-B及其两个变位剪切体VEGF-B167、VEGF-B186mRNA的表达,并评估其临床意义.方法:应用荧光定量PCR法检测肝癌、癌旁、乙型肝炎肝硬化、慢性乙型肝炎及正常肝组织中总VEGF-B、VEGFB167及VEGFB186mRNA的表达量,与患者的临床病理资料做相关性分析,并结合随访资料做生存分析.结果:总VEGF-B及VEGF-B186mRNA在各组组织中表达量无统计学差异;VEGF-B167mRNA在各组间表达量有统计学差异,正常组表达最高,乙型肝炎肝硬化组最低(252.59vs168.00,P=0.005),肝癌组中VEGF-B167的表达量明显高于慢性乙型肝炎组和乙型肝炎肝硬化组(234.13vs170.72,168.00;P=0.043,P<0.01).依据VEGF-B表达高低,将肝癌患者分组,VEGF-B167低表达组患者的总体生存率较高表达组和无差异组有升高趋势(P=0.097),而VEGF-B167低表达组患者术后无瘤生存率明显高于其他两组(P=0.023).肝癌组织中总VEGF-B、VEGF-B167及VEGF-B186的表达与患者性别、年龄、病毒感染、肝硬化、Child-Pugh分级、AFP值、肿瘤数目、肿瘤大小、血管侵犯、TNM分期及肿瘤分化之间相关性无统计学意义.结论:VEGF-B参与了肝癌的发生发展过程,VEGF-B167在此过程中的作用较VEGF-B186更重要.VEGF-B167低表达患者预后较好,术后总生存率和无瘤生存率较高.AIM： To detect the mRNA expression of vascular endothelial cell growth factor B （VEGF-B） and its splice isoforms VEGF-B167 and VEGF-B186 in different types of liver lesions and to assess theirclinical significance. METHODS： The mRNA expression of VEGF-B, VEGF-B167 and VEGF-B186 in liver cancer, cancer-adjacent tissue, liver cirrhosis, chronic hepatitis B, and normal liver tissue was detected by quantitative real-time reverse transcription-polymerase chain reaction （RT-PCR）. The correlation of VEGF-B, VEGF-B167, and VEGF-B186 expression with the clinical pathological data of patients was analyzed, and survival analysis was performed using follow-up data. RESULTS： There were no statistical differences in the expression levels of total VEGF-B and VEGF-B186 among each group, while the expression level of VEGF-B167 mRNA was significantly different among each group, highest in the normal group but lowest in liver cirrhosis group （252.59 vs 168.00, P = 0.005）. The expression level of VEGF-B167 mRNA was significantly higher in the liver cancer group than in the chronic hepatitis B group （234.13 vs 170.72, 168.00, both P 0.05）. The overall survival rate and tumor-free survival rate of patients with low VEGF-B167 expression tended to be higher than those of patients with high VEGF-B167 expres-sion （P = 0.097, 0.023）. The expression levels of total VEGF-B, VEGF-B167 and VEGF-B186 had no significant correlation with sex, age, virus infection, liver cirrhosis, Child-Pugh score, AFP score, tumor number or size, vascular invasion, TNM stage, or tumor differentiation. CONCLUSION： VEGF-B is involved in hepatocar-cinogenesis and VEGF-B167 plays a more important role than VEGF-B186 in this process. Patients with low VEGF-B167 expression have good prognosis since their postoperative overall survival rate and tumor-free survival rate are higher than those with high VEGF-B167 expression.

关 键 词：肝细胞癌 血管内皮生长因子B MRNA 实时定量聚合酶链式反应 

分 类 号：R735.7[医药卫生—肿瘤]