三氧化二砷联合干扰素抑制人表皮癌细胞株A431的生长  

The effect of As_2O_3 combined with INF on human epidermal carcinoma cells A431 in vitro

作　　者：耿春杰 张国强[2] 高顺强[2] 李美洲[2] 

机构地区：[1]河北省邢台市第三医院皮肤科,054000 [2]河北医科大学第四医院皮肤科

出　　处：《河北医药》2011年第4期488-490,共3页Hebei Medical Journal

基　　金：河北省医学科学研究重点课题(编号:08361)

摘　　要：目的探讨三氧化二砷(As2O3)联合干扰素(INF)在不同浓度、不同作用时间对人表皮癌细胞细胞株A431增殖的抑制作用,筛选两药应用的最佳作用时间和剂量。方法通过对A431细胞常规传代培养,采用MTT比色分析法检测不同浓度As2O3、INF及二者联合对人A431细胞增殖抑制的影响;采用流式细胞分析术,检测人A431细胞的细胞周期变化和凋亡的情况。结果与阴性对照组比较,As2O3在1～10μmol/L浓度范围内对A431细胞有明显的增殖抑制作用,在24～72h对人A431细胞的抑制作用随着浓度的增加而增强,且以10μmol/L浓度组作用72h的抑制率最高(P<0.01)。与阴性对照组比较,INF在一定浓度和时间范围内对A431细胞作用也有相似结果。As2O3能诱导细胞凋亡,随着时间的延长和浓度的增加凋亡细胞数目增加。结论 As2O3和INF均对人A431细胞有明显的抑制作用,且呈现剂量依赖效应关系和时间依赖效应关系,As2O3和INF均能使S期延滞,并提高S期细胞比例,两药联合有协同效应。As2O3诱导A431细胞凋亡的作用显著,但INF能强化As2O3诱导A431细胞凋亡的作用。Objective To investigate the inhibition effect of arsenic trioxide combined with interferon on the growth of epidermal carcinoma cells at different concentrations and different time in order to select the optimized action time and dose of the combination of the two drugs.Methods Through cell culture with human epidermal carcinoma cells A431 in vitro,the inhibition effects of arsenic trioxide combined with INF at different concentrations on proliferation of A431 cells were detected by MTT.The apoptosis rate and the changes of cell cycle in human A431 cells were detected by FCM.Results The results of MTT showed that arsenic trioxide and INF respectively inhibited the proliferation of A431 cells in a dose-dependent and time-dependent manner.The combination of arsenic trioxide with INF inhibited the growth of A431 cells,and the inhibitory rate of the combination application was significantly higher than that of arsenic trioxide only,there was a significant difference between arsenic trioxide and INF（P〈0.01）.There was a synergistic effect of arsenic trioxide and INF combination application.The synergistic effects of arsenic trioxide and INF on inducing apoptosis of A431 cells was more obvious,as compared with that of using simple arsenic trioxide or INF.Conclusion The arsenic trioxide and INF has inhibition effect on the growth of A431 cells in vitro respectively in a dose-dependent and time-dependent manner.The arsenic trioxide and INF can result in the arrearage of S phase of cell growth,and can increase the ration of S phase,and there is a synergistic effect of arsenic trioxide and INF combination application.The induction effect of arsenic trioxide on apoptosis of A431cells is quite obvious,nut INF can enhance the induction effect of arsenic trioxide on apoptosis of A431cells.

关 键 词：AS2O3 干扰素 表皮癌 A431细胞 凋亡 

分 类 号：R739.5[医药卫生—肿瘤]