rhHSP70对食管癌细胞RNA转染的脐血树突状细胞诱导CTL抗肿瘤功能的影响  

Effect of rhHSP70 on anti-tumor activity of CTL induced by human cord blood dendritic cells transfect with RNA of esophageal carcinoma cells

作　　者：王健[1] 张永泽[1] 潘丽兰[1] 史玉[1] 门金娥[1] 郑海萍[1] 张向阳[1] 田珂[1] 

机构地区：[1]河北工程大学附属医院中心实验室,邯郸056038

出　　处：《疑难病杂志》2011年第1期45-47,共3页Chinese Journal of Difficult and Complicated Cases

基　　金：河北省科技研究与发展计划资助项目(No.064072225D)

摘　　要：目的研究重组人热休克蛋白70(rhHSP70)联合食管癌细胞RNA转染脐血树突状细胞(DCs)对细胞毒性T淋巴细胞(CTL)特异性抗肿瘤作用的影响。方法分离脐血单个核细胞,经rhSCF、rhGM-CSF和IL-4诱导和食管癌RNA转染形成成熟DCs,加入rhHSP70,检测DCs表面标志及DCs诱导的T细胞增殖、CTL杀伤活性。结果食管癌RNA转染后DCs高表达抗原提呈分子、协同刺激分子和黏附分子,对T细胞的促增殖作用和CTL杀伤活性显著增强(P<0.01),rhHSP70能显著促进DCs功能。结论 rhHSP70具有增强食管癌细胞RNA转染的DCs对T细胞的促增殖和CTL杀伤活性的作用。Objective To investigate the effect of rhHSP70 and human cord blood dendritic cells（DCs） transfect with RNA of esophageal carcinoma on anti-tumor activity of CTL.Methods The RNA of T.Tn cells were prepared by Trizol reagent.The cord blood monocytes were cultured to produce DCs with rhSCF,rhGM-CSF and IL-4.The DCs were collected and transected with tumor cell RNA.CpG ODN was added in culture medium to enhance the DCs vaccine.The mixed lymphocyte reaction（MLR） and cytotoxicity of CTL to T.Tn cells was assayed by MTT colorimetry.Results Compared with pretransfect stage,DCs transfect with RNA of esophageal carcinoma cells showed more MHC-Ⅰ,MHC-Ⅱ,CD54,CD 80 and CD86（P0.01）.Compared with immature DCs,mature DCs transfect with tumor RNA enhanced tumor cytolytic activities of T lymphocyte.rhHSP70 could significantly increase the number of molecules on surface of activated T lymphocyte.Conclusion The vaccine of cord blood DCs with adjuvant of rhHSP70 can provide effective and specific way for immunological therapy of esophageal carcinoma.

关 键 词：树突状细胞 重组人热休克蛋白70 T细胞毒性 食管肿瘤 脐血 

分 类 号：R735.1[医药卫生—肿瘤]