CD24在大肠癌组织中的表达及其对癌细胞增殖的影响  

The Expression of CD24 and its Effect on Tumor Cell Proliferation in the Colorectal Cancer

作　　者：王伟飞[1] 王新颖[2] 岳辉[1] 毛正果[1] 陈培生[1] 

机构地区：[1]南方医科大学第三附属医院消化内科,510630 [2]南方医科大学南方医院消化内科,510515

出　　处：《实用癌症杂志》2011年第1期37-41,44,共6页The Practical Journal of Cancer

摘　　要：目的探讨CD24对大肠癌SW480细胞的增殖作用。方法应用免疫组织化学方法,检测大肠癌及癌旁正常组织中CD24的表达情况,用pcDNA3.1(+)载体构建CD24的表达质粒[pcDNA3.1(+)-CD24],应用半定量RT-PCR和流式细胞术,检测CD24 mRNA和蛋白水平的表达情况,应用CCK-8法,检测SW480及转染重组质粒的细胞体外增殖情况。结果 CD24在大肠癌组织中的染色定位于细胞膜和细胞质,膜表达和质表达阳性率分别为34.9%和89.6%,质表达水平与肿瘤病理分级和分期呈正相关性;成功地构建了pcDNA3.1(+)-CD24表达质粒并瞬时转染SW480细胞,质粒转染组在转染48、72和96 h时,细胞活性较阴性对照组和空白对照组明显增强(P<0.01)。结论 CD24在大肠癌组织中呈高表达,而且可促进大肠癌细胞的体外增殖作用,提示CD24在大肠癌发生发展中起重要作用。Objective To explore the effect of CD24 on proliferation ability of the SW480 cells.Methods CD24 protein in the colorectal cancer tissue and adjacent normal mucosa was evaluated by immunohistochemical analysis.A CD24 expression plasmid[（pcDNA3.1（＋）-CD24）]was constructed using pcDNA3.1（＋） vector,the expression level of CD24 mRNA and protein were measured by semi-quantitative reverse transcription polymerase chain reaction（RT-PCR） and Flow cytometry,Cell proliferation was assessed by CCK-8 assay after transfecting CD24 expression plasmid into SW480 cells.Results CD24 showed membranous and cytoplasmic immunostainings in colorectal tissue,and the positive rate was respectively 34.9% and 89.6%.There was a significantly positive correlation between cytoplasmic CD24 expression and tumor stage and grade.CD24 expression plasmid was constructed successfully,CCK-8 assay showed the proliferation of the SW480 cells overexpressed with CD24 was more quick than SW480 cells transfected with the vector alone and the parental SW480 cells（P0.01）.Conclusion The CD24 expression was higher in the colorectal cancer tissue and overexpression of CD24 induced proliferation in SW480 cells.These data implicate that CD24 gene may play an important role in colorectal tumorigenesis.

关 键 词：CD24基因 大肠癌 增殖 

分 类 号：R735.34[医药卫生—肿瘤]