肖氏生物液中总糖及总酚的含量测定  

Determination of Total Sugar and Total Hydroxybenzene in Xiaoshi Biological Fluids

作　　者：张一[1] 肖海涛[1] 郝小燕[1] 

机构地区：[1]贵阳医学院,贵州贵阳550004

出　　处：《食品研究与开发》2011年第1期90-93,共4页Food Research and Development

基　　金：贵州省科技厅工业攻关项目(黔科合GY字[2009]3053)

摘　　要：建立紫外分光光度法测定肖氏生物液中总糖及总酚的含量。分别采用苯酚-硫酸法和Folin-酚法测定其总糖和总酚含量。总糖在5.27μg/mL～26.33μg/mL内线性良好r,=0.999 3,平均含量为635.64 mg/g,平均回收率为101.11%,RSD=2.19%(n=6);总酚在1.21μg/mL～7.24μg/mL内线性良好r,=0.999 7,平均回收率为100.79%,RSD=2.19%(n=6)。所建立的方法简便、可靠、重现性好、专属性强,可用于肖氏生物液的质量控制。To establish a method for the determination of total sugar and total hydroxybenzene in Xiaoshi biological fluids.Phenol-sulfuric method and Folin-Denis method were used for the determination of content of total sugar and total hydroxybenzene in Xiaoshi biological fluids,respectively.The total sugar showed a good linear relationship in the range of 5.27 μg/mL-26.33 μg/mL,r=0.999 3,the average content was 635.64 mg/g.The average recovery was 101.11 %,RSD=2.19 %（n=6）;the total hydroxybenzene showed a good linear relationship in the range of 1.21 μg/mL-7.24 μg/mL,r=0.999 7,the average recovery was 100.79 %,RSD=2.19 %（n=6）.Established methods are simple,convenient,reliable and can be used to control the quality of Xiaoshi biological fluids.

关 键 词：肖氏生物液 总糖 总酚 含量测定 

分 类 号：R286.0[医药卫生—中药学]