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作 者:李雪[1,2] 徐迎春[1] 李永荣 李晓储[3,2]
机构地区:[1]南京农业大学园艺学院,南京210095 [2]南京绿宙薄壳山核桃科技有限公司 [3]江苏省林业科学研究院
出 处:《林业科技开发》2011年第1期70-73,共4页China Forestry Science and Technology
基 金:江苏省林业三项工程项目"薄壳山核桃优良新品种选育与示范推广"(编号:JSX(2009)01);南京科技计划项目"薄壳山核桃新品种选育及快繁技术研究"(编号:200901002)
摘 要:通过TTC染色法测定4个薄壳山核桃品系Mahan(‘马汉’)、Western(‘威斯登’)、Shoshoni(‘肖肖尼’)、‘钟山25’的花粉分别在室温、4℃和-20℃等不同贮藏条件下活力的变化。研究结果表明,-20℃条件下花粉活力贮藏时间最长,到140天时花粉活力仍在50%左右。以花粉活力保持在50%为标准,室温条件下Western和‘钟山25’贮藏时间最长(8天);4℃下Western花粉贮藏时间最长(21天);-20℃下,4个品系贮藏天数都在84天以上,其中Western、‘钟山25’贮藏时间最长,达140天。The dynamics of pollen viability under different storage condition of four Carya illinoensis varieties were assayed by TTC so as to provide technical support for the artificial pollination.The dynamics of pollen viability under room temperature,4℃ and-20℃ storage condition of four varieties were assayed.Results showed that with the storage prolonging,pollen viability gradually decreased.-20℃ storage had the longest pollen viability,which could reach 50% at 140th day.With the pollen viability at 50% as a standard,under room temperature,Western and 'Zhongshan 25' had longest storage time of 8 days;Under 4℃ pollen storage time Western was the longest storage time of 21 days;When storage under-20℃,the storage time of the 4 viabilities were more than 84 days,in which Western,'Zhongshan 25' had longest storage time,up to 140 days.
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