HepG2和L5178Y细胞彗星试验检测甲磺酸甲酯和环磷酰胺遗传毒性的比较研究  

Genetic Toxicity Detection of Methanesulfonate(MMS) and Cyclophosphamide(CP) by Comet Assay with HepG2 and L5178Y Cells

作　　者：段佳[1] 陈祥贵[1] 帅培强[2] 饶夙[1] 刘振平[1] 

机构地区：[1]西华大学生物工程学院,四川成都610039 [2]四川出入境检验检疫局,四川成都610041

出　　处：《西华大学学报（自然科学版）》2011年第1期19-20,75,共3页Journal of Xihua University:Natural Science Edition

基　　金：国家质检总局重大项目(2008IK257);四川省重点学科建设项目(SZD0803-09-0);西华大学人才培养项目(0325136;ZG0720506)

摘　　要：为了探索HepG2细胞彗星试验的应用价值,采用HepG2和L5178Y细胞彗星试验检测了直接致突变物甲磺酸甲酯(MMS)和间接致突变物环磷酰胺(CP)的遗传毒性。结果表明,HepG2细胞彗星试验不需要外源活化系统就可检测出MMS和CP的遗传毒性,而L5178Y细胞彗星试验需要外源S9活化系统才能检测到CP的遗传毒性。因此,HepG2细胞彗星试验在检测间接致突变物时不需要外源活化系统,操作简便,有广泛应用价值。In order to explore the application value of HepG2 cells in the comet assay,the genetic toxicity of the direct mutagens methyl methanesulfonate（MMS） and indirect mutagens cyclophosphamide（CP） were evaluated respectively by comet assay with HepG2 or with L5178Y cells.The results show that comet assay with HepG2 cell can detect the genetic toxicity of MMS and CP in absence of exogenous S9activation system while the comet assay with L5178Y cells needs exogenous S9 activation system to detect the genotoxicity of CP.Therefore,the comet assay with HepG2 cell has a simpler procedure and wider application.

关 键 词：彗星试验 HEPG2 L5178Y 甲磺酸甲酯 环磷酰胺 

分 类 号：R361[医药卫生—病理学]