新霉素耐药基因(Neo^R)的整合与表达并不改变人乳癌细胞(MDA)的原有生物学特性  

Biological characteristics of human breast cancer cell line (MDA) expressing neomycin resistance gene (Neo R)

作　　者：郭斯启[1] 奚永志[1] 宋三泰[1] 金荔[1] 陈兴国[1] 孔繁华[1] 

机构地区：[1]军事医学科学院附属医院免疫学研究室,北京100039

出　　处：《中国病理生理杂志》1999年第8期729-731,共3页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金!资助(No .39370272)

摘　　要：目的：通过对比ＭＤＡ－ ＮｅｏＲ 与ＭＤＡ 人乳癌细胞的生物学特征，阐明原核标志基因ＮｅｏＲ 的长期稳定整合与表达是否对乳癌细胞原有的生物学特性产生影响。方法：对已建立且证实表达ＮｅｏＲ 基因＞ ６ 个月的７ 株ＭＤＡ－ＮｅｏＲ 细胞系进行Ｇ４１８ 抗性、表面抗原、细胞倍增时间、细胞周期、肿瘤细胞集落形成及裸鼠体内成瘤能力的检测。结果：ＭＤＡ－ ＮｅｏＲ 细胞对高浓度的Ｇ４１８ 仍产生抗性并表达Ｎｅｏ Ｒ 基因；其细胞倍增时间、Ｓ 期分数、肿瘤细胞体外集落形成率与ＭＤＡ 无明显区别（ Ｐ＞ ０－０５） ；角蛋白ＣＫ１９ 与表面膜抗原ＥＭＡ 的表达在两组是等同的；并且ＭＤＡ－ ＮｅｏＲ ７ 细胞系在裸鼠体内的成瘤能力与ＭＤＡ 细胞也是相似的。结论：外源标志基因ＮｅｏＲ 的长期稳定整合与表达对ＭＤＡAIM: To compare the biological characters of MDA cell line with MDA-Neo R cell lines, those of expressing neomycin resistance gene (Neo R). METHODS: Several biological characters were identified. They are G418 (geneticin) resistance, the expression of cell membrane antigens, double-cell time, the fraction of cell cycle phase, the numbers of tumor colony formation in vitro and the tumor formation ratio of nude mice in vivo. RESULTS: MDA-Neo R cells cultured in vitro for six months are still endurable for G418(6×10 5 U/L); The expression of CK19 (cytokeratin 19) and epithelial membrane antigen detected by (flow cytometer ) are negative; Double-cell time, the ratio of cell in S phase, and the colony formation numbers in vitro are insignificant statistically ( P >0.05) between MDA and MDA-Neo R cell lines; The tumor formation ratio of nude mice in vivo of MDA and one of MDA-Neo R cell lines are 1/4 and 2/5 respectively, have no difference. CONCLUSION: The integration and expression of hetrogenious Neo R gene do not make any changes in the characters of MDA.

关 键 词：新霉素 乳腺癌 MDA-Neo^R 耐药基因 

分 类 号：R737.902[医药卫生—肿瘤]