聚N-异丙基丙烯酰胺硅胶键合相RP-HPLC检测化妆品中防腐剂  

Determination of preservatives in cosmetics by RP-HPLC using poly(N-isopropylacrylamide)-graftedsilica as stationary phase

作　　者：董伟 范旭 曹光群 崔莹 

机构地区：[1]江南大学化学与材料工程学院,无锡214122

出　　处：《分析仪器》2011年第1期24-28,共5页Analytical Instrumentation

基　　金：教育部留学回国人员启动基金(1044130201080100);江南大学青年科学基金资助项目(10400052210730)

摘　　要：以聚N-异丙基丙烯酰胺硅胶键合相为固定相,建立了化妆品中苯甲酸、对羟基苯甲酸甲酯、对羟基苯甲酸乙酯、对羟基苯甲酸丙酯和对羟基苯甲酸丁酯5种防腐剂的反相高效液相色谱测定方法。以不锈钢色谱柱(250mm×4.6mm,5μm)作为分离柱,以甲醇-磷酸盐缓冲液(55:45,pH=2.5)为流动相,流速0.5mL/min,紫外检测波长254nm。样品先通过氧化铝柱进行分离处理,馏出液用于色谱分析。校正曲线的线性范围分别为:40～1000mg/L(苯甲酸),4～100mg/L(对羟基苯甲酸甲酯,一乙酯,一丙酯),8～200mg/L(对羟基苯甲酸丁酯)。回收率96.6%～106.9%,相对标准偏差0.60～1.70%。A reversed-phase high-performance liquid chromatographic （RP-HPLC） method was developed using poty（N-isopropylacrylamide）-grafted silica as stationary phase for the determination of preservatives benzoic acid and, methyl-, ethyl, propyl-, and n-butyl p-hydroxybenzoate in cosmetics. The separation was performed on a stainless steel column （250mm×4.6mm, 5um）. The methanol-phosphate buffer （55：45, pH=2.5） was used as mobile phase with a flow rate of 0.5mL/min and the detection was at 254nm. The sample was firstly pretreated on an aluminium oxide column. The eluent was analyzed by RP-HPLC. The calibration curves were linear in the ranges of 40-1000mg/L for benzoic acid, 4-100mg/L for methyl-, ethyl-and propyl-p-hydroxybenzoate, and 8 - 200ug/L for n-butyl p-hydroxybenzoate. The recoveries were 96.6 %-- 106.9%, and RSDs were 0.60%-1.70%.

关 键 词：聚N-异丙基丙烯酰胺硅胶键合相 反相高效液相色谱 化妆品 防腐剂 苯甲酸 对羟基苯甲酸 酯类 

分 类 号：TQ658[化学工程—精细化工]