反相高效液相色谱法测定川楝子中阿魏酸含量  

Determination of Ferulic Acid in Melia Toosendan by RP-HPLC

作　　者：杨冠华[1] 李阳[1] 张丽[1] 许良[1] 

机构地区：[1]内蒙古民族大学化学化工学院,内蒙古通辽028042

出　　处：《广州化工》2011年第3期11-12,15,共3页GuangZhou Chemical Industry

基　　金：国家自然科学基金项目(No:30760299)

摘　　要：建立测定川楝子中阿魏酸含量的高效液相色谱法。采用VP-ODS C18（250 mm×4.6 mm,5μm）色谱分析柱;流动相为甲醇-1%冰醋酸（25：75）;检测波长：320 nm;流速：0.8 mL/min。结果阿魏酸进样量在0.25~2.50μg范围内与峰面积呈良好的线性关系（r=0.999 6）,平均加样回收率为99.38%,RSD=1.18%（n=6）。该方法简便、准确、可靠,可作为川楝子中阿魏酸的定量分析方法。HPLC method for the determination of ferulic acid in Melia toosendan was established.The chromatographic separation was performed on a VP-ODS C18 column(250 mm×4.6 mm,5 μm),using methanol-1.0% glacial acetic acid(25:75) as mobile phase.The detection wavelength set at 320 nm and the flow rate was 0.8 mL/min.The results showed that the linear range of ferulic acid was 0.25~2.50 μg,the average recovery rate was 99.38%(r=0.999 6),and RSD was 1.18%(n=6).This method was simple,accurate and reliable.It could be used as the method to determine the contents of ferulic acid in Melia toosendan.

关 键 词：HPLC 川楝子 阿魏酸 

分 类 号：O657.72[理学—分析化学]