兔VX2肾癌模型的构建及其外周血细胞因子的变化  

Changes of the peripheral blood cytokines in rabbit with VX2 renal carcinoma

作　　者：王玉忠[1] 郭宏骞[1] 刘光香[1] 

机构地区：[1]南京医科大学鼓楼临床医学院泌尿外科,江苏南京210008

出　　处：《江苏大学学报（医学版）》2011年第1期23-25,45,F0003,共5页Journal of Jiangsu University：Medicine Edition

基　　金：江苏省卫生厅指导性项目(Z200712);南京市卫生局一般项目(YKK08060);南京市科技发展计划项目(201001104)

摘　　要：目的:通过观察外周血细胞因子的变化,探讨兔VX2肾癌模型建立后免疫系统的变化及其机制。方法:40只新西兰大白兔随机分为两组。肿瘤模型组采用外科手术组织块接种法建立兔VX2肾癌模型,正常对照组植入自体肾周脂肪组织。术后14天采取两组兔外周血,采用双抗体夹心ELISA法检测外周血中的干扰素-γ和白介素-2、6、10含量。结果:与对照组比较,模型组外周血中的干扰素-γ、IL-2降低(P<0.05),IL-6和IL-10增高(P<0.05)。结论:VX2肿瘤种植后可抑制实验兔的免疫系统,表现为外周血干扰素-γ、IL-2含量降低,IL-6和IL-10含量增高。Objective： To observe the changes of cytokines in peripheral blood of rabbit VX2 renal carcinoma and investigate the mechanism of the immune system changes.Methods： Forty rabbits were randomly divided into two groups,for tumor model group,VX2 tumor pieces were implanted into the renals,using surgical tissue inoculation to construct rabbit VX2 renal carcinoma model,the other was the control group,implanted autogenetic adipose tissue.Then the changes of interferon-γ and interleukin 2,6,10 were observed.Results： After VX2 tumor transplantation,tumor model group,compared with the control group,IFN-γ and IL-2 decreased,IL-6 and IL-10 increased（P0.05）.Conclusion： After constructing rabbit VX2 renal carcinoma model,it can cause immunosuppression,expressed as IFN-γ,IL-2 decreased,IL-6 and IL-10 increased.

关 键 词：VX2肿瘤 肾癌 细胞因子 兔 干扰素 白细胞介素 

分 类 号：R737.11[医药卫生—肿瘤]