曲古霉素A对人肾细胞癌Caki-1细胞体外生长的影响  

Influence on the growth by trichostatin A in human renal cell carcinoma Caki-1 cells in vitro

作　　者：姚宏伟[1] 孙浩[2] 何鸿保[1] 唐爱国[2] 

机构地区：[1]江苏大学临床医学院,江苏镇江212001 [2]江苏大学附属医院泌尿外科,江苏镇江212001

出　　处：《江苏大学学报（医学版）》2011年第1期46-49,共4页Journal of Jiangsu University：Medicine Edition

摘　　要：目的:观察组蛋白去乙酰化酶抑制剂曲古霉素A(trichostatin A,TSA)体外对人肾细胞癌Caki-1细胞生长情况、乙酰化组蛋白H3的水平以及基因P21cip1/waf1mRNA表达的影响,并探讨其可能的作用机制。方法:四甲基偶氮唑盐(MTT)比色法检测TSA对Caki-1细胞生长的影响;蛋白质印迹法检测TSA处理后Caki-1细胞乙酰化组蛋白H3水平的变化;实时荧光定量PCR检测TSA处理后Caki-1细胞P21cip1/waf1mRNA表达水平的变化。结果:TSA对Caki-1细胞生长有显著的抑制作用,呈明显的剂量、时间依赖性。TSA能明显提高Caki-1细胞乙酰化组蛋白H3的水平,促进P21cip1/waf1mRNA表达。结论:TSA对人肾细胞癌Caki-1细胞生长有抑制作用,机制可能与其提高Caki-1细胞乙酰化组蛋白H3的水平,促进P21cip1/waf1的表达有关。Objective： To investigate the effect of trichostatin A（TSA）,a histone deacetylace inhibitor,on the growth of human renal cell carcinoma Caki-1 cells in vitro,the levels of acetylated histone H3 and the mRNA expression of P21cip1/waf1 gene,then to explore the mechanism involved.Methods： MTT assay was used to examine the effect of TSA on the growth of Caki-1 cells.The levels of acetylated histone H3 after TSA treatment was evaluated by western blot.The mRNA expression of P21cip1/waf1 was analyzed by qRT-PCR.Results： TSA obviously inhibited the growth of Caki-1 cells in a time and dose dependent manner.TSA obviously enhanced the levels of acetylated histone H3 and induced the expression of P21cip1/waf1 mRNA.Conclusion： TSA can induce the growth arrest of human renal cell carcinoma Caki-1 cells,which might be related to increase the levels of acetylated histone H3 and induce the expression of P21cip1/waf1.

关 键 词：曲古霉素A 肾细胞癌 组蛋白 乙酰化 P21CIP1/WAF1 

分 类 号：R737.11[医药卫生—肿瘤]