核桃青皮在体外清除自由基的活性研究  

Study on capacities of free radical effects by Walnut peel in vitro

作　　者：吕海宁[1] 折改梅[1] 郭志琴[1] 折冬梅[2] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]中国农业科学院植物保护研究所,北京100193

出　　处：《华西药学杂志》2011年第1期32-33,共2页West China Journal of Pharmaceutical Sciences

摘　　要：目的研究核桃青皮体外清除自由基的活性。方法采用清除2,2-二苯基苦味酰基苯肼基(DPPH)和2,2'-连氮基-双-(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)自由基的方法,对核桃青皮乙醇提取物不同极性部位进行清除自由基能力的综合评价。结果石油醚、氯仿、乙酸乙酯、正丁醇、水萃取核桃青皮清除DPPH自由基的IC50分别为27.0、19.0、5.6、8.8、44.0 mg.L-1,清除ABTS自由基的IC50分别为114.0、82.6、13.6、32.0、302.0 mg.L-1。清除自由基的能力依次为:乙酸乙酯部位>正丁醇部位>氯仿部位>石油醚部位>水部位,其中,乙酸乙酯部位清除DPPH和ABTS自由基的能力高于同浓度的Vit C和Vit E。结论核桃青皮提取液的乙酸乙酯、正丁醇萃取部位具有很好的清除自由基活性,前者活性强于后者,是天然抗氧化物质的良好来源。OBJECTIVE To study the capacitis of free radical effects by extracts from walnut peel（Juglans regia）.METHODS The capacitis of free radical effects by different extracts from walnut peel were measured by DPPH assay and ABTS assay.RESULTS All extracts（petroleum ether,chloroform,ethyl acetate,n-butanol,aqueous solution） from walnut peel showed strong capacitis of free radical effects The IC50 of DPPH free radical effects were 27.0,19.0,5.6,8.8,44.0 mg·L-1,respectively.The IC50 of scavenging ABTS free radicals were 114.0,82.6,13.6,32.0 and 302.0 mg·L-1,respectively.The capabilitis of free radical effects in five solvents were ethyl acetaten-butanolchloroformpetroleum etheraqueous solution.The capabilitis of DPPH and ABTS free radical effects by ethyl acetate extracts was stronger than Vit C and Vit E.CONCLUSION The ethyl acetate extracts and the n-butanol extracts exhibited strong capacitis of free radical effects.Walnut peel proves to be a good resource of natural antioxidant product.

关 键 词：核桃青皮 不同极性部位 体外 清除自由基 

分 类 号：R96[医药卫生—药理学]