HPLC法检测止咳平喘类中成药掺入化学药品的方法  

Discussion on Method of Separation and Detection of Cough and Asthma Medicines Mixed with Chemicals by HPLC

作　　者：张裕民 

机构地区：[1]山东省菏泽市药品检验所,菏泽274014

出　　处：《中国药事》2010年第12期1213-1215,1242,共4页Chinese Pharmaceutical Affairs

摘　　要：目的建立HPLC法分离检测止咳平喘类中成药掺入化学药品的方法。方法高效液相色谱法,色谱柱为Agilent C18;流动相为A:甲醇-乙腈(1∶1),B:取甲酸铵0.6g,加冰乙酸2.0mL,加水至2000mL使溶解,即得;A-B(45∶55)。柱温为35℃;检测波长为225nm;流速为0.8mL.min-1。结果方法稳定、重复性好、回收率高、准确可靠。结论本方法可以作为高效液相色谱法分离检测止咳平喘类中成药掺入化学药品的方法。Objective To find a way of separation and detection of cough and asthma medicines mixed with chemicals.Methods HPLC was used.column： Agilent C18.Mobile phase： A： methanol-acetonitrile（1∶1）;B： take ammonium 0.6g and glacial acetic acid 2.0mL,add water to 2000mL,allow ammonium dissolved;A-B（45∶55）.Column temperature： 35℃,detection wavelength： 225nm,flow rate： 0.8mL·min-1.Results The method is stable,reproducible,highly recovery,accurate and reliable.Conclusion HPLC can be used as the method of separation and detection of cough and asthma medicines mixed with chemicals.

关 键 词：HPLC 分离检测 止咳平喘类中成药 掺入 化学药品 

分 类 号：R917[医药卫生—药物分析学]