低浓度Aβ1-42单体／寡聚体及一氧化碳供体对SN56细胞存活率的影响  

Effect of low concentration Aβ1-42 monomer/oligomers and CORM-2 on livability of SN56 cells

作　　者：金曼[1] 吕佩源[1] 李玲[1] 董艳红[1] 岳洪娟[1] 李金玲[1] 范呜玥[1] 

机构地区：[1]河北省人民医院神经内科,石家庄050051

出　　处：《中华行为医学与脑科学杂志》2010年第12期1096-1098,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：基金项目：河北省国际科技合作行划项目（09396427D）

摘　　要：目的观察低浓度Aβ1-42单体／寡聚体对胆碱能SN56细胞存活率的影响以及不同浓度CORM-2的作用？方法将相同细胞浓度的SN56细胞培养在96孔细胞培养板中并将细胞分为对照组、Aβ1-42组、Aβ1-42＋CORM-250μM组及A[3142＋CORM-2100μM组：Aβ1-42组三列细胞分别在10nM、100nM及1μMAβ1-42单体／寡聚体的环境中培养.Aβ1-42＋CORM-250μM组和A13。＋CORM-2100μM组细胞除每孔分别含50μM及100μMCORM-2外，其余培养条件与Aβ1-42组相同。对照组不加任何处理因素。培养3d后，用MTF法比较不同组别细胞存活率。结果Aβ1-42组细胞存活率分别为（79．73±0．94）％、（67．99±0．79）％、（60．42±0．62）％，随Aβ1-42浓度增加而下降。Aβ1-42＋CORM-250μM／100μM组细胞存活率分别为（75．15±0．096）％、（63．20±0．17）％、（55．33±0．19）％；（73．20±0．27）％、（64．34±0．11）％、（54，17±0．12）％，均低于单纯Aβ1-42组；并且不同CORM-2浓度之间，降低细胞存活率的作用存在差异。结论低浓度Aβ1-42单体／寡聚体可降低SN56细胞的存活车，并且浓度越高，效果越明显；不同浓度CORM-2均可使SN56细胞的存活率下降．且作用强度存在差异。Objective ＇ro observe the effect of low concentration Aβ1-42 nmnomer/oligomers and CORM-2 in different concentration on livabilitv of SN56 cells. Methods SN56 cells were cuhured in the 96-well plate with uniform concentration, and were divided into control group, Aβ1-42 group, Aβ1-42 ＋ CORM-2 50μM group, and Aβ1-42＋ CORM-2 100 o.M group. Three lines of cells in Aβ1-42, group were cuhnred in the surroundings of 10 nM, 100nM and 1 μMAβ1-42monomer/oligomers, respectively. Aβ1-42 ＋ CORM-2 50μM group and Aβ1-42 ＋ CORM-2 100μM group had the same cuhure condition as group Aβ1-42,except contain 50μM, and 100μM CORM-2, respectively. Control group didnt have any effect factor. Three days later,the fivabilily of different groups was compared with MTT method. Results The livability of group Aβ1-42 with the increasing concentration of Aβ1-42 was （ 79.73 ± 0.94 ） % , （ 67.99 ± 0.79 ） % , （ 60.42 ± 0.62 ） % , respectively. The higher the concentration of Aβ1-42 was,the lower the livability of SN56 cell was. The livability of group Aβ1-42 ＋ CORM-2 501.zM/100txM was（75.15 ±0.096）% ,（63. 20 ± 0. 17）%, （55. 33 ±0. 19）% ; （73.20 ±0. 27）%, （64. 34 ±0. 11 ）%, （54. 17± 0. 12）% , respectively. Both were Dower than group Aβ1-42 And different CORM-2 concentration had discrepancy in the ability of decreasing the cell livability. Conclusion Low concentration of Aβ1-42 can reduce the livability of SN56 cells, and higher concentration has more significant effect; CORM-2 in different concentration both can decrease the livahility of SN56 cells,and there is a discrepancy in the intensity.

关 键 词：阿尔茨海默瓬 AΒ1-42 一氧化碳 SN56细胞 存活率 

分 类 号：R749.16[医药卫生—神经病学与精神病学]