抑郁模型大鼠行为学及海马体积变化的动态研究  

Dynamic research about the change of the behavior and hippocampal volume of rat depressive model

作　　者：罗一烽[1] 王冬青[1] 李月峰[1] 孙维斌[1] 何伟[1] 殷瑞根[1] 许云飞[1] 王铮超[1] 赵亮[1] 

机构地区：[1]江苏大学临床医学院影像系、附属医院影像科,镇江212013

出　　处：《中华行为医学与脑科学杂志》2010年第12期1102-1104,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：基金项目：江苏省卫生厅科技计划资助项目（H200951）;镇江市科技计划项目（SH2009017）

摘　　要：目的探讨大鼠抑郁模型的行为学及海马体积在MRI上的动态变化及两者的相关性。方法30只SD大鼠根据行为学评分后，随机分为对照组和抑郁组，每组I5只。运用慢性不可预知应激及孤养模式建立抑郁模型，应激共8周。于建模开始前及每周末进行大鼠体质量测量、液体消耗及Morris水迷宫试验，并于实验开始前及每两周末在高场强MRI上测量每组大鼠各侧海马体积。结果各项行为学指标表明建模成功并呈动态变化。与对照组相比，建模第4周，抑郁组大鼠左侧海马体积显著减小[抑郁组（37．13±2．40）mm3，对照组（39．05±2．05）mm3，t=2．36，P〈0．05]，右侧无明显变化；第6周时，右侧也明显减小[抑郁组（37．85±2．11）mm3，对照组（39．44±2．10）mm3，t=2．07，P〈0．05]，左侧继续减小；总体减小幅度左侧大于右侧（左：15％，右：8％）。同时发现行为学指标与海马体积缩小具有明显相关性。结论大鼠抑郁模型的行为学改变及海马体积呈动态变化，且具有一定的规律性。Objective To explore the dynamic change and correlation of the behavior and hippocampa[ volumes by MRI of rat depressive model. Methods Based on the evaluation of ethology, thirty SD rats were randomly and equally divided into control group and model group which was exposed to chronic unpredictable mild stress for 8 weeks. The body weight,fluid consumption test and Morris water maze test were done at the beginning and per week while the bilateral hippocampal volumes of every rat were measured by high field MRI at the beginning and per two weeks. Results Based on the behavioral data, the rat depressive model was established successfully, and （：hanged dynamically. On 4th week, the left hippocampal volumes of depressive rat were significantly reduced （ model group （ 37.13 ± 2.40 ） mm3, control group （ 39.05 ± 2.05 ） mm3, t = 2.36, P 〈 0.05 ） while the right ones did not. On 6th week, the right ones began to reduce significantly （ model group （37.85 ± 2.11 ） mm3 , control group（39.44±2.10） mm3 , t =2.07, P〈0.05 ） while the left one still reduced,but the extent of the left＇ s reduction was bigger than the right （left：15% ,right ：8% ）. And there was apparent correlation between the behavior and the reduction of hippocampal volumes. Conclusion The behavior and hippocampal volumes of rat depressive model changes dynamically, and there was a regularity of them.

关 键 词：抑郁模型 行为学 磁共振成像 海马 

分 类 号：R749.4[医药卫生—神经病学与精神病学]