辐射诱发细胞HPRT基因位点突变频率的研究  

STUDY ON THE FREQUENCY OF RADIATION INDUCED HPRT SITE MUTATION AND ITS DOSE EFFECT RELATIONSHIP

作　　者：王海燕[1] 张连珍[1] 

机构地区：[1]中国辐射防护研究院,太原030006

出　　处：《辐射防护》1999年第6期466-469,共4页Radiation Protection

摘　　要：本文采用多核细胞检测法（ＣＢ法），研究了６０Ｃｏ γ射线照射离体人血所诱发的体细胞ＨＰＲＴ基因位点的突变频率。实验结果表明，０～５０ Ｇｙ γ射线可诱发ＨＰＲＴ基因位点突变，ＨＰＲＴ基因位点突变的频率随照射剂量增加而升高，突变频率Ｙ（１０－ ３） 与照射剂量Ｄ（Ｇｙ）间可拟合为Ｙ＝The frequency of HPRT site mutation induced in vitro by 60 Co γ rays in human blood cells and its dose effect relationship were investigated using a more simple, rapid and sensitive polynuclear cell assay. The results show that the frequency of HPRT site mutation induced by 0~5.0 Gy of γ rays was significantly higher than that for control. The frequency of HPRT site mutation is increased lineally with doses and could be represented in a lineal model Y =0 903+0 869 D . (

关 键 词：HPRT位点 基因突变频率 Γ射线 辐射诱发 细胞 

分 类 号：R818.02[医药卫生—放射医学]