原发性胆汁性肝硬化患者中β-arrestin 1表达增高及其意义  

β-arrestin 1 Increased in Patients with Primary Biliary Cirrhosis

作　　者：黄元兰[1] 陈燕[1] 周晔[1] 陈孙孝[1] 邓安梅[1] 仲人前[1] 

机构地区：[1]第二军医大学长征医院实验诊断科,全军临床免疫中心,上海200003

出　　处：《现代检验医学杂志》2010年第6期11-14,共4页Journal of Modern Laboratory Medicine

基　　金：本课题受国家自然科学基金：（30671840.30770997,30772017,30972730）,上海科委基金：（09JC2405400,07XD14013.08QH14001）资助.

摘　　要：目的 探讨原发性胆汁性肝硬化（PBC）患者中β-arrestin 1的表达及其临床意义.方法 采用实时荧光定量PCR检测60例PBC患者、60例乙肝后肝硬化患者和60例健康体检者PBMCs中β-arrestin 1 mRNA水平,采用免疫印迹（western-blotting）技术检测β-arrestin 1蛋白表达水平.结果 PBC患者PBMCs中β-arrestin 1 mRNA的表达显著高于健康对照组和疾病对照组（P＜0.01）,而疾病对照组和健康对照组PBMCs中β-arrestin 1 mRNA的表达差异无统计学意义（P＞0.05）,并且β-arrestin 1表达水平与Mayo risk score正相关（Pearson r2=0.593 5,P＜0.001）.PBC患者PBMCs中β-arrestin 1蛋白水平显著高于健康对照组和疾病对照组（P＜0.01）,而疾病对照组和健康对照组比较差异无统计学意义（P＞0.05）.结论 PBC患者中β-arrestin 1表达显著增高,提示β-arrestin 1在PBC发病机制中可能起着重要作用,有可能作为PBC疾病进程的一个新的免疫状态标志.Objective To investigate expression of β-arrestin 1 in patients with primary biliary cirrhosis （PBC） and the clinical significances. Methods PBMCs from 60 PBC patients,60 liver cirrhosis after hepatitis B virus infection and 60 healthy controls were collected. β-arrestin 1 mRNA level was evaluated by reverse-transcription（RT） reat-time（RT） fluorescence quantitative polymerase chain reaction （FQ-PCR） ,the protein was assayed by western-blotting. Results The level of β-arrestin 1 mRNA in PBC patients increased significantly（P〈0.01） and correlated to Mayo risk score positively （Pearson r2 =0. 593 5,P〈0. 001）. Meanwhile ,the expression of β-arrestin 1 protein in PBC was higher markedly than that in healthy controls. Conclusion β-arrestin 1 could be involved in the pathogenesis of PBC and as a marker of immunological status in PBC.

关 键 词：原发性胆汁性肝硬化 Β-ARRESTIN 1 荧光定量PCR 免疫印迹 

分 类 号：R575.22[医药卫生—消化系统] R392.11[医药卫生—内科学]