去甲二氢愈创木酸联合肿瘤坏死因子相关凋亡诱导配体对胰腺癌细胞PCNA-1的抑制作用  

Inhibitory effect of nordihydroguaiaretic acid combined with TNF - related apoptosis - inducing ligand on the growth of pancreatic cancer cells

作　　者：张文[1] 刘志苏[1] 高卉[2] 

机构地区：[1]武汉大学中南医院普外科,430071 [2]咸宁学院临床医学院外科教研室

出　　处：《临床外科杂志》2010年第12期814-816,共3页Journal of Clinical Surgery

摘　　要：目的 探讨脂氧合酶抑制剂去甲二氢愈创木酸（nordihydroguaiaretic acid,NDGA）联合肿瘤坏死因子相关凋亡诱导配体（tumor necrosis factor-related apoptosis inducing ligand,TRAIL）对胰腺癌细胞的抑制作用.方法 MTT法测定NDGA、TRAIL及NDGA联合TRAIL作用于PCNA-1细胞后的细胞生存率,同时,用流式细胞技术测定细胞凋亡率,Western b1ot检测caspase-3的表达水平的变化.结果 各组给药组与对照组比较均能不同程度抑制PCNA-1细胞增值并诱导其凋亡作用,且caspase-3表达上调;NDGA与TRAIL联用明显增强诱导凋亡作用.结论 NDGA与TRAIL联用可明显增强PCNA-1细胞抑制效果和诱导凋亡作用,其诱导凋亡的机制可能与其上调caspase-3表达有关.Objective To investigate the inhibitory effect of nordihydroguaiaretic acid （NDGA） in combination with TNF - related apoptosis - inducing ligand（TRAIL） on the growth of pancreatic cancer ceils. Methods MTT methods was used for detecting the survival rate of PCNA - 1 ceils treated with NDGA, TRAIL alone or combined. The apoptosis rate of PCNA - 1 ceils was tested by flow cytometry（FCM）. The expression of caspase -3 was measured by Western blotting. Results Compared with that of the control cells, the proliferation of PCNA -1 cells was much inhibited and the apoptosis was greatly induced in all the treatment groups, and the expression of caspase - 3 was significantly up - regulated. The combined use of NDGA and TRAIL could induce the apoptosis of PCNA - 1 ceils. Conclusion Combined use of NDGA and TRAIL could obviously inhibit the growth of PCNA - 1 cells and induce their apoptosis ,which was associated with the up - regulation of expression of caspase - 3.

关 键 词：胰腺癌 脂氧合酶抑制剂 肿瘤坏死因子相关凋亡诱导配体 凋亡 

分 类 号：R735.9[医药卫生—肿瘤]