二烯丙基二硫化物对人子宫内膜癌HEC-1-B细胞增殖抑制和凋亡诱导作用  

Proliferation inhibition and apoptosis induction in human endometrial carcinoma cell line HEC-1-B by diallyl disulfide

作　　者：李京敏[1] 刘蛟[1] 王安冉[1] 

机构地区：[1]滨州医学院人体解剖与组织胚胎学研究所,烟台市264003

出　　处：《滨州医学院学报》2010年第6期440-442,444,共4页Journal of Binzhou Medical University

基　　金：滨州医学院科技计划(BY2008KJ27);滨州医学院大学生科技创新活动基金项目(BY2009DKCX23)

摘　　要：目的研究二烯丙基二硫化物(DADS)对人子宫内膜癌HEC-1-B细胞增殖和凋亡的影响。方法 MTT法检测DADS对HEC-1-B细胞的增殖抑制作用,采用AnnexinV/PI双标记流式细胞术检测DADS对HEC-1-B细胞的凋亡诱导作用;Hoechst-33528染色,观察细胞凋亡形态。结果用100、200、400μmol/L DADS处理HEC-1-B细胞24、48 h,在100μmol/L剂量即可抑制细胞的增殖,随着剂量和时间的增加,DADS对细胞的抑制作用明显增强。DADS对HEC-1-B细胞的增殖抑制存在时间-剂量依赖关系。用200μmol/LDADS处理48 h后,流式细胞术检测细胞凋亡率达40.91%。Hoechst-33528染色可见细胞出现明显的凋亡形态学改变。结论 DADS对HEC-1-B细胞具有增殖抑制并诱导凋亡的作用,提示DADS对子宫内膜癌的治疗可能具有重要价值。Objective To investigate the impact of DADS on the proliferation inhibition and apoptosis induction in human endometrial carcinoma cell line HEC-1-B.Methods MTT assay was used to detect the proliferation inhition of DADS on HEC-1-B cells.AnnexinV/PI staining was performed to quantify apoptotic cells by flow cytometry.Hoechst-33528 stanining was employed to observe the apoptosis morphology.Results After DADS treatment at the concentration of 100,200,400 μmol/L for 24,48 h,the proliferation could be inhibited at the concentration of 100 μmol/L.The growth inhibition of DADS was strengthened in a dose-time-dependent manner.After DADS treatment for 48 h at the concentration of 200 μmol/L,the ratio of apoptosis cells was 40.91% by fluorescence microscopy and flow cytometry.Obvious apoptotic morphology changes was observed by Hoechst-33528 stanining.Conclusion DADS can induce proliferation inhibition and apoptosis in HEC-1-B cells,which indicates that DADS may be therapeutically useful to endometrial carcinoma chemotherapy.

关 键 词：二烯丙基二硫化物 HEC-1-B细胞 细胞增殖 细胞凋亡 

分 类 号：R737.33[医药卫生—肿瘤]