手持式光电比色仪快速定量测定铅、铬  

A portable photoelectric method for the rapid determination of Pb^(+2) and Cr^(+6)

作　　者：王红勇[1] 房彦军[1] 周焕英[1] 宁保安[1] 高志贤[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《中国卫生检验杂志》2010年第12期3174-3176,3180,共4页Chinese Journal of Health Laboratory Technology

基　　金："863"计划课题(2007AA06Z419);天津市科技计划项目(092CKFSH02700);国家科技重大专项(2009ZX09308-006);唐山市科学技术研究与发展计划(09110202A-3-2)

摘　　要：目的:检测试剂和手持式光电比色仪联用的方法,实现对样品中铅、铬的现场快速定量检测。方法:在酸性条件下,六价铬与二苯碳酰二肼作用,生成紫色络合物(544 nm);在碱性条件下,铅与水杨基荧光酮形成三元配合物(560 nm)。生成的络合物呈色深浅分别与铅、铬含量成一定相关性,于手持式光电比色仪实现定量测定。结果:铬浓度在0.0～2.0 mg/L范围内线性关系良好(r=0.9970);方法的最低检出量为0.2μg、加标回收率为88%101%;铅浓度在0.0～10.0 mg/L范围内线性关系良好(r=0.9959);方法的最低检出量为0.5μg、加标回收率为75%88%。结论:通过将显色试剂固定在纸片上,操作过程中纸片替代检测试剂,使得铬的检测更为便捷;试剂-光电比色仪方法测定铅、铬的方法具有操作简便、快速、适合现场定量检测,值得推广应用。Objective：To establish a rapid and quantitative method for the determination of Pb＋2 and Cr＋6 in samples on the spot by the testing agent combining with a portable photolometer.Methods： In acidic condition,Cr＋6 can react with diphenyl carbohydrazide and generate one kind of purple complex compound（max=544 nm）.In alkaline condition,Pb＋2 can react with salicylflyuorene and generate ternary complexes（max=560 nm）.There is a good linear relationship between the shades of the compound′s color and the concentration of Pb＋2/ Cr＋6 which could be quantitatively determined by portable photolometer.Results： The linear working range of Pb＋2 was 0.0～10.0 mg/L（r=0.9959） with the detection limit of 0.5 μg and the recovery was 75%～88% respectively.And the linear working range of Cr＋6 was 0.0～2.0 mg/L（r=0.9970） with the detection limit of 0.2 μg and the recovery was 88%～101%.Conclusion： By means of fixing the chromogenic reagent on the scrip which substituted the detection reagent in the operation,the detection of Cr＋6 is more convenient.This method reagents combined with portable photolometer for the determination of Pb＋2 and Cr＋6 is simple,rapid and suitable for the quantitative field detection and worth of application and extension.

关 键 词：光电比色仪 铬 铅 定量检测 

分 类 号：TS207.51[轻工技术与工程—食品科学]