大肠杆菌和肺炎克雷伯氏杆菌表面抗原的研究  

STUDY OF THE SURFACE ANTIGEN OF E COLI AND KL PNEUMONIAE

作　　者：熊焰[1] 苟琳[1] 王印[1] 张和民[2] 何廷美[2] 李德生[2] 胡大明[2] 

机构地区：[1]四川农业大学动物科技学院,雅安625014 [2]中国保护大熊猫研究中心,卧龙623006

出　　处：《畜牧兽医学报》1999年第6期558-561,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：四川省教委重点科研资助

摘　　要：采用热抽提和ＴｒｉｔｏｎＸ１００ 处理２ 种方法提取大肠杆菌ＥＬ１ 株和肺炎克雷伯氏杆菌ＥＬ２ 株表面抗原，经胃蛋白酶处理、ＳＤＳＰＡＧＥ 及免疫印迹分析表明，ＥＬ１ 、ＥＬ２ 株主要表面抗原耐热，对胃蛋白酶不敏感，分子量分别是２０ＫＤ、３３ＫＤ 和３６ＫＤ、４３ＫＤ，其抗原成分是多糖或多糖磷脂和蛋白质的复合物。二菌株表面抗原具有各自菌种的特征，可作为菌种分类鉴定的重要指标。此外，热抽提法提取细菌表面抗原的效果优于Ｔｒｉｔｏｎ Ｘ１００。The surface antigens of E coli(Strain EL 1) and KL Pneumoniae (Strain EL 2)were extracted by heat treatment or by triton X 100 treatment and were treated with proteiase k The antigens,after analysis by polyacryamide gel electrophoresis and immunoblotting,have been shown to be resistant to heat and nonsensitive to proteiase k The molecular weights of the antigens were 20KD、33KD and 36KD、43KD respectively They were composed of polysaccharid or the complex of polysaccharid phosphatied and protein Because the surface antigens in two strains were not common,they had the species character and could be considered a key index in classified identification of species Moreover,the effect of heat extraction in extracted resistance heat surface antigen of bacteria was better than Triton X 100

关 键 词：大肠杆菌 肺炎 克雷伯氏杆菌 表面抗原 SDS-PAGE 

分 类 号：S852.61[农业科学—基础兽医学]