毛葡萄原生质体分离与纯化研究  

Protoplast Isolation and Purification of Vitis quinquangularis Rehd

作　　者：袁彬[1,2] 潘学军[1,2] 

机构地区：[1]贵州省果树工程技术研究中心,贵阳550025 [2]贵州大学喀斯特山地果树资源研究所,贵阳550025

出　　处：《西南大学学报（自然科学版）》2010年第12期97-101,共5页Journal of Southwest University(Natural Science Edition)

基　　金：贵州省优秀科技教育人才省长专项资金[黔省专合字(2006)10号];贵州省优秀青年科技人才培养对象专项资金资助[黔科合人字(2009)02号]

摘　　要：以毛葡萄愈伤组织、悬浮培养细胞、无菌苗幼叶为材料,研究了毛葡萄原生质体的分离纯化方法及影响因素.结果表明,毛葡萄原生质体分离材料以悬浮细胞和愈伤组织最好.毛葡萄愈伤组织在0.5 mol/L甘露醇中预处理90 min,CPW+13%甘露醇+2%纤维素酶+0.25%果胶酶+0.5%离析酶的酶液中酶解10 h,其原生质体产量最高可达6.24×106个/g,活力为86.83%.To establish a good system of protoplast isolation and purification for Vitis quinquangularis Rehd,calli,suspension cells and tube-plant leaves of V.quinquangularis were used as explants to study the influencing factors for protoplast isolation and purification.The results showed that the most suitable materials were calli and suspension cells for protoplast isolation.Pretreating the calli of V.quinquangularis with 0.5 mol/L mannitol and zymolyzing them in an enzyme liquid of CPW＋0.5 mol/L mannitol ＋ 2% cellulose ＋ 0.25% pectinase ＋ 0.5% macetating enzyme for 90 min gave the highest yield of protoplast of 6.24×106/g with a viability of 86.83%.

关 键 词：毛葡萄 愈伤组织 原生质体 分离 纯化 

分 类 号：Q949.756.3[生物学—植物学]