缺血对体外培养星形胶质细胞凋亡及细胞周期的影响  

Effects of Ischemia on Apoptosis and Cell Cycles of Cultured Astrocytes

作　　者：余勇飞[1] 许康[1] 李平[1] 谢敏杰[2] 喻志源[2] 朱舟[2] 王伟[2] 

机构地区：[1]湖北省新华医院神经内科,武汉430015 [2]华中科技大学同济医学院附属同济医院神经科,武汉430030

出　　处：《神经损伤与功能重建》2011年第1期15-18,共4页Neural Injury and Functional Reconstruction

摘　　要：目的:研究缺血干预不同时间对体外培养的星形胶质细胞(Ast)凋亡及细胞周期的影响。方法:分离新生大鼠皮质,获得细胞悬液,通过培养、分离和传代方法纯化Ast,经体外缺血条件(氧及血清剥夺,低糖)干预3、6、12和24h后收集细胞,PI染色。流式细胞仪检测每个样品单个细胞DNA含量,测算每个样品中处于凋亡及各周期细胞的百分比。结果:对照组与各缺血干预组进行组间比较,凋亡百分比无显著性差异。观察期内Ast始终未出现凋亡。缺血3、6、12hAstS期及G2/M期百分比较对照组显著增高,缺血6h达最高值。结论:Ast对缺血有一定的耐受性,缺血早期Ast反应性增生。Objective：To study the effects of ischemia on apoptosis and cell cycle of cultured astrocytes.Methods：Cell suspension was obtained by dissociating the cerebral cortex of neonatal rats.Astrocytes were purified by culture,detachment and passages.Astrocytes were treated with in vitro ischemic condition（deprivation of oxygen and serum,low glucose）,harvested from ischemia-treated cultures at 3,6,12 and 24 h,and then stained with PI（Propidine Iodide）.Contents of DNA in each sample were measured by flowcytometry.The percentage of apoptotic cells and the cell cycle were quantified.Results：Control and ischemia groups showed no significant differences in the percentage of apoptosis cells.No apoptotic astrocytes was found throughout the course of ischemia.The percentages of S and G2/M phases in astrocytes were increased significantly at 3,6 and 12 h after ischemia when compared with the controls,which peaked at 6 h.Conclusion：Astrocytes are relatively resistant to ischemia.Astrocytes become reactive under ischemia.

关 键 词：缺血 星形胶质细胞 凋亡 细胞周期 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.02[医药卫生—临床医学]